Competitive Binding of Novel Cyanine Dye AK3-5 and Europium Coordination Complexes to DNA

  • Olga Zhytniakivska Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0001-9554-0090
  • Anna Zabrudska Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-3611-8188
  • Uliana Tarabara Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7677-0779
  • Kateryna Vus Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine http://orcid.org/0000-0003-4738-4016
  • Valeriya Trusova Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7087-071X
  • Galyna Gorbenko Department of Physics and Technology, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine http://orcid.org/0000-0002-0954-5053
  • Atanas Kurutos Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria https://orcid.org/0000-0002-6847-198X
  • Todor Deligeorgiev Faculty of Chemistry and Pharmacy, Sofia University ‘‘St. Kliment Ohridski’’, Sofia, Bulgaria
Keywords: trimethine cyanine dye, europium coordination complexes, antitumor drug, DNA

Abstract

The present study was undertaken to assess the applicability of the novel trimethine cyanine dye AK3-5 as a competitive ligand for the antitumor agents, Eu(III) coordination complexes (EC), in the DNA-containing systems, using the displacement assay as an analytical instrument. The analysis of fluorescence spectra revealed a strong association of AK3-5 with nucleic acids, with the strength of interaction being higher for the double stranded DNA, compared to the single-stranded RNA. The binding parameters of the cyanine dye have been determined in terms of the McGhee & von Hippel neighbouring site-exclusion model and a classical Langmuir model. The AK3-5 association constant in the presence of DNA was found to be equal to 5.1×104 M-1, which is consistent to those of the well-known DNA intercalators. In turn, the binding of the cyanine to the RNA was characterized by a significantly lower association constant ( ~ 3.4×103 M-1) indicating either the external or “partially intercalated” binding mode. The addition of the europium complexes to the AK3-5-DNA system was followed by the fluorescence intensity decrease, with a magnitude of this effect being dependent on the EC structure. The observed fluorescence decrease of AK3-5 in the presence of europium complexes V7 and V9 points to the competition between the cyanine dye and antitumor drugs for the DNA binding sites. The dependencies of the AK3-5-DNA fluorescence intensity decrease vs. europium complex concentration were analyzed in terms of the Langmuir adsorption model, giving the values of the drug association constant equal to 5.4×104 M-1and 3.9×105 M-1 for the europium complexes V7 and V9, respectively. A more pronounced decrease of the AK3-5 fluorescence in the presence of V5 and V10 was interpreted in terms of the drug-induced quenching of the dye fluorescence, accompanying the competition between AK3-5 and Eu(III) complexes for the DNA binding sites. Cumulatively, the results presented here strongly suggest that AK3-5 can be effectively used in the nucleic acid studies and in the dye-drug displacement assays.

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Published
2019-10-01
Cited
0 article
How to Cite
Zhytniakivska, O., Zabrudska, A., Tarabara, U., Vus, K., Trusova, V., Gorbenko, G., Kurutos, A., & Deligeorgiev, T. (2019). Competitive Binding of Novel Cyanine Dye AK3-5 and Europium Coordination Complexes to DNA. East European Journal of Physics, (3), 63-70. https://doi.org/10.26565/2312-4334-2019-3-08