THE EFFECT OF EARLY POSTNATAL OVERNUTRITION ON PROOXIDANT-ANTIOXIDANT BALANCE AND NO-SYNTHASE ACTIVITY IN OLD RATS TISSUES
Keywords:
overnutrition, prooxidant-antioxidant balance, thyroid status, NO-synthase activity, rats
Abstract
The peculiarities of changes of prooxidant-antioxidant balance, thyroid status and NO-synthase activity in tissues of 20-month-old rats after overnutrition during first month after birth were investigated. It was found out that body weight, thyroxine concentration, lipid hydroperoxides content and NO-synthase activity in experimental rats were higher than in control ones. At the same time Se-dependent glutathione peroxidase activity was significantly decreased in liver of experimental rats. It is assumed that prooxidant-antioxidant system and thyroid state may be ones of the key links of the mechanism determining rats surviving in conditions of overnutrition.
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References
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5. Mitochondria and mitochondrial nitric oxide synthase alterations participate in energetical dysbalance, aging and age-related diseases / R. Dzurik, Z. Krivosikova, K. Stefikova [et al.] // Bratisl. Lek. Listy. – 2006. – Vol. 107, № 11-12. – P. 405–411.
6. Yu B. P. Dietary restriction downregulates free radical and lipid peroxide production: plausible mecha-nism for elongation of life span / B.P. Yu, B.O. Lim, M. Sugano // J. Nutr. Sci. Vitaminol. (Tokyo). – 2002. – Vol. 48, № 4. – P. 257–264.
7. Белостоцкая Л.И. Влияние трех различных гипокалорийных диет на окислительное фосфорилиро-вание и активность ферментативной антиоксидантной системы в митохондриях печени крыс / Л.И. Белостоцкая, В.Н. Дзюба, Ю.В. Никитченко // Успехи геронтол. – 2008. – Т. 21, № 2. – С. 235–239.
8. Стан ферментативної антиоксидантної системи в тканинах щурів за умов надмірного харчування / Ю.В. Нікітченко, В.М. Дзюба, А.С. Попович [та ін.] // Мед. хімія. – 2009. – Т. 11, № 4. – С. 159–161.
9. Ohkawa H. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction / H. Ohkawa, N. Ohahi, K. Jadi // Anal. Biochem. – 1979. – Vol.. 95, № 2. – P. 351–358.
10. Asakawa T. Coloring condition of thiobarbituric acid test for detecting lipid hydroperoxides / T. Asa-kawa, S. Matsushita // Lipids. – 1980. – Vol. 15, № 3. – P. 137–140.
11. Ланкин В.З. Ингибирование переокисления липидов и детоксикация липоперекисей защитными ферментными системами (супероксиддисмутаза, глутатион-пероксидаза и глутатион-редуктаза) при экспериментальном злокачественном росте / В.З. Ланкин, С.М. Гуревич // Докл. АН СССР. – 1976. – Т. 226, № 3. – С. 705–708.
12. Ravin H. A. Rapid test for hepatolenticular degeneration / H. A. Ravin // Lancet. – 1956. – Vol. 1. – P. 7267–7271.
13. An assay method for nitric oxide synthase in crude samples by determining product NADP+ / W. Wang, N. Inoue, T. Nakayama [et al.] // Anal. Biochem. – 1995. – Vol. 227, № 2. – P. 274–280.
14. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids / L. C. Green, D. A. Wagner, J. Glogowski [et al.] // Anal. Biochem. – 1982. – Vol. 126. – P. 131–138.
15. Miller S. I. Protein determination for large numbers of samples / S. I. Miller // Anal. Chem. – 1959. – Vol. 31, № 5. – P. 964–966.
16. Окислительный стресс. Прооксиданты и антиоксиданты / Е. Б. Меньщикова, В.З. Ланкин, Н.К. Зенков [и др.]. – М. : Фирма «Слово», 2006. – 556 с.
17. Inhibition of nitric oxide synthase enhances superoxide activity in canine kidney / D.S. A. Majid, A. Nishiyama, K.E. Jackson [et al.] // Am. J. Physiol. Regul. Integr. Comp. Physiol. – 2004. – Vol 287. – P. R27–R32.
How to Cite
Nikitchenko, Y., Dzyuba, V., PopovichА., SheremetА., & Bondar, V. (1). THE EFFECT OF EARLY POSTNATAL OVERNUTRITION ON PROOXIDANT-ANTIOXIDANT BALANCE AND NO-SYNTHASE ACTIVITY IN OLD RATS TISSUES. The Journal of V. N. Karazin Kharkiv National University, Series "Medicine", (19), 12-16. Retrieved from https://periodicals.karazin.ua/medicine/article/view/6930
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Fundamental research
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