Calorimetriс and spectroscopic investigation of proflavine - salmon testes DNA binding
Keywords:
calorimetry, spectrophotometry, DNA, proflavine, complexationAbstract
The interaction of proflavine with salmon testes DNA is investigated by differential scanning calorimetry
(DSC) and spectrophotometry in solutions with ionic strength I=0,02 M and I=0,1 M. We have applied the
Scatchard formalism to analyze the spectrophotometric data. The thermodynamic melting parameters of DNA
in the complexes with proflavine are obtained from DSC data. It is shown that proflavine forms two different
types of complexes on DNA. The thermodynamic properties of double helix in these complexes are different.
The complete thermodynamic profile for proflavine intercalation is obtained. Binding parameters for
intercalation determined from calorimetric and spectrophotometric data are in a good agreement.
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