Dielectric properties of human cord blood serum after long-term low temperature storag

Authors

  • О. V. Lipina Institute of problem of cryobiology and cryomedicine Author
  • О. А. Gorobchenko V.N. Karazin Kharkov National University Author
  • О. А. Nardid Institute of problem of cryobiology and cryomedicine, NAS of Ukraine Author
  • О. S. Yasunova V.N. Karazin Kharkov National University Author
  • О. Т. Nikolov V.N. Karazin Kharkov National Universit Author

Keywords:

cord blood serum, low temperature storage, dielectric permittivity

Abstract

In the article the results of studying of the influence of long-term low temperature storage on dielectric
properties of human cord blood serum (HCBS) are presented. In the study human cord blood serum
diluted by physiological solution in ratio 1:2 was used. The purpose of the study was found out by
gamma-irradiation and the method of UHF-dielectrometry the influence of long-term storage of HCBS
samples at -20°С and -196°C on water state change and, consequently, on a structure of
biomacromolecules in HCBS. The serum was gamma-irradiated after unfreezing in order to detect hidden
damages of protein components. It was shown that freezing down to -196°С and storage in liquid nitrogen
during four years have smaller influence on water state in HCBS and, consequently, on structure of
macromolecules in HCBS, and therefore it is more preferable in comparison with freezing and storage at
-20°С

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Author Biographies

  • О. V. Lipina, Institute of problem of cryobiology and cryomedicine

    Pereyaslavskaya Str., 23, Kharkov 61015, Ukraine

  • О. А. Gorobchenko, V.N. Karazin Kharkov National University

    Svobody Sq., 4, Kharkov 61022, Ukraine

  • О. А. Nardid, Institute of problem of cryobiology and cryomedicine, NAS of Ukraine

    Pereyaslavskaya Str., 23, Kharkov 61015, Ukraine

  • О. S. Yasunova, V.N. Karazin Kharkov National University

    Svobody Sq., 4, Kharkov 61022, Ukraine

  • О. Т. Nikolov, V.N. Karazin Kharkov National Universit

    Svobody Sq., 4, Kharkov 61022, Ukraine

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Issue

Vol. 2 No. 30 (2013): Біофізичний вісник

Section

Action of physical agents on biological objects

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How to Cite

Dielectric properties of human cord blood serum after long-term low temperature storag. (2015). Biophysical Bulletin, 2(30). https://periodicals.karazin.ua/biophysvisnyk/article/view/2320

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