Temperature effect on erythrocyte distribution by spherical index
Keywords:
erythrocyte, shape, spherical index, coolingAbstract
The effect of temperature in the range of 37 - 3°C on human erythrocyte shape was investigated. Methods of light microscopy and determination of cell distribution density by spherical index (SI) were used. The investigation shows that the part of cells with small SI (1-1,3) dramatically increases in the temperature range from 15 to 8°C and has a maximum at 12-10°C, corresponding to appearance and growth of additional maximum in the erythrocyte distribution density curves. The cell density in the range of large SI (>1,7) decreases with temperature fall below physiological one and takes values, as a rule, less than 5% at the temperatures under 20°C. By light microscopy method, there was shown that when reaching the temperature of 15°C a part of cells dramatically changes their shape by “discocyte – stomatocyte” transition, which leads to the destruction of preliminarily formed rouleaux.
Downloads
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
