Encapsulation of europium chelates into lipid vesicles

Authors

  • E. V. E.V. Pakhomova V.N. Karazin Kharkov National University Author
  • O. K. Kutsenko V.N. Karazin Kharkov National University Author
  • G. P. Gorbenko V.N. Karazin Kharkov National University Author
  • V. M. Trusova V.N. Karazin Kharkov National University Author
  • T. Deligeorgiev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia Author
  • A. Vasilev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia Author
  • S. Kaloianova Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia Author
  • N. Lesev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia Author

Keywords:

europium chelates, doxorubicin, liposomes, fluorescence quenching

Abstract

The partitioning of europium chelates (EC) between the aqueous and lipid phases was studied. The
possibility of coencapsulation of two anticancer drugs (doxorubicin and europium complex) into lipid
vesicles was evaluated. Partition coefficients obtained by equilibrium dialysis technique indicate that EC
can be effectively entrapped by the lipid phase of liposomes. Measurement of intrinsic fluorescence of
doxorubicin suggests that this agent is located mainly in the inner aqueous phase of liposomes. EC were
found to quench the fluorescence of Laurdan and pyrene. The effect of doxorubicin on incorporation of
europium complexes into lipid bilayer was demonstrated to depend on chemical structure of chelates.

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Author Biographies

  • E. V. E.V. Pakhomova, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkov, 61077

  • O. K. Kutsenko, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkov, 61077

  • G. P. Gorbenko, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkov, 61077

  • V. M. Trusova, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkov, 61077

  • T. Deligeorgiev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

    Bulgaria

  • A. Vasilev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

    Bulgaria

  • S. Kaloianova, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

    Bulgaria

  • N. Lesev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

    Bulgaria

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Issue

Vol. 1 No. 26 (2011): Біофізичний вісник

Section

Cell biophysics

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How to Cite

Encapsulation of europium chelates into lipid vesicles. (2015). Biophysical Bulletin, 1(26). https://periodicals.karazin.ua/biophysvisnyk/article/view/2707

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