The estimation of transmembrane potentials single hepatocytes by means of fluorescent probes
Keywords:
mitochondrial membrane potential, fluorescent probes, hepatocytesAbstract
In work in comparative aspect characteristics of the binding with rats’ hepatocytes newly synthesized dialkyl (С4 and С18) derivatives of the probe, JС-1 were investigated. It has been established, that С4-derivatives are characterized similarly with JС-1 dynamics of accumulation in cells. In that time total intensity of cell fluorescence dyed by С4-variant and aggregate and monomer forms ratio was lower, than at the using JС-1 in the same concentration. At an estimation of dynamics of burning out С4-derivatives in a mode of continuous supervision of cells, it was shown that it is more photosensitive in comparison with the initial form. From all investigated variants of a probe intensity of fluorescence of cells and aggregate and monomer forms ratio was the lowest at dyeing of cells by С18 derivatives. At an estimation of parameters of fluorescence under influence of protonophore FCCP, sensitivity to changes in mitochondrial potential is shown only for the initial form of the dye.
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