Indicator dye behavior in the system glucose oxidase + glucose

Authors

Keywords:

glucose biosensor, glucose oxidase, indicator dyes

Abstract

Using the absorption spectroscopy technique the group of indicator dyes including bromocresol purple, phenol red, neutral red, auramine O and acridine orange has been examined from the viewpoint of the dye applicability to the design of optical glucose biosensors. The absorbance changes of bromocresol purple, phenol red and neutral red observed in the glucose oxidase + glucose system were interpreted ui terms of the dye sensitivity to the pH reduction occurring while glucose is catalytically oxidized. The arguments in favor of neutral red interaction with glucose oxidase are obtained.

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Author Biographies

  • G. P. Gorbenko, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkov, 61077

  • Ye. A. Domanov, V.N. Karazin Kharkov National University

     4 Svobody Sq., Kharkov, 61077

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Published

2018-06-25

Issue

Section

Methods of biophysical investigations

How to Cite

Indicator dye behavior in the system glucose oxidase + glucose. (2018). Biophysical Bulletin, 2(13), 116-119. https://periodicals.karazin.ua/biophysvisnyk/article/view/12470

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