Peculiarities of interaction calcium and copper ions with pyrimidine bases in frozen water solutions
Keywords:
nucleic acids, pyrimidine nitrogen bases, calcium and copper ions, low temperature secondary ion mass spectrometryAbstract
Interaction of nucleic acid pyrimidine bases m13Ura, m11Thy, Cyt with calcium and copper ions in frozen water solutions was studied using low temperature fast atom bombardment mass spectrometric technique. Inspection of systems with varied concentrations of bases and chlorides of the metals have shown that base-metal associates recorded in the mass spectra are connected with finely dispersed structure of the eutectic of the frozen samples. It was revealed that the rate of the interactions and types of the associates formed are dependent on the divalent metal type. Calcium ions showed higher yields of associates with the bases in the solid frozen systems in comparison with dilute water solutions. Calcium ions produced along with electrostatically bound complexes covalent ones by substitution of a proton of a base, while copper ions produced only non-covalent low-abundant associates. In the case of thymine base which is prone to self-association, the distinctions between calcium and copper interactions were preserved on the level of the base dimers.
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