Approach to analyse kinetics of erythrocyte hemolysis
Abstract
The hemolytic stability of erythrocytes is studied under various pathological conditions, including ischemia and complications after myocardial infarction. A large set of parameters are used to describe hemolysis, among which the duration of stages, the rate of stage, the percentage of hemolysed cells and others. Diagnostic interpretation of such results is difficult and is of little purpose for clinicians. The characteristics of hemolysis often are the kinetic parameters. A method for analyzing the kinetics of hemolysis is proposed, which makes it possible to obtain additional information on the distribution of erythrocytes by their hemolytic resistance. The method is easily implemented and allows obtaining statistically significant results suitable for automated diagnosis. The developed methodology was applied to the analysis of acid hemolysis of healthy volunteers and patients with cardiovascular pathology. The described approach to the analysis of the process of hemolysis of erythrocytes made it possible to reveal changes in the resistance of erythrocytes to acid-induced hemolysis in people with coronary heart disease and to detect a change in the ratio of the number of erythrocytes with different life span in the circulation.
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Mahapatra D. Recent advances in statistical data and signal analysis: Application to real world diagnostics from medical and biological signals / D. Mahapatra, K. Agarwal, R. Khosrowabadi, D.K. Prasad // Comput. Math. Methods Med. – 2016. – V. 2016. – Article ID 1643687.
Review of point-of-care testing and biomarkers of cardiovascular diseases in emergency and prehospital medicine / P.G. Claret, X. Bobbia, C. Roger [et al.] // Acta Cardiol. – 2015. – V. 70 (5). – P. 510-515.
Kinetics of hemolysis of normal and abnormal red blood cells in glycerol-containing media. / A. Sauer, T. Kurzion, D. Meyerstein, N. Meyerstein. // Biochim Biophys Acta. – 1991. – V. 1063 (2). – Р. 203-211.
Chmura A. The kinetics of hemolysis as an indicator of spherocytosis. / A. Chmura, G. Paździor , M. Langner // Cell Mol Biol Lett. – 2002. – V. 7 (2). – Р. 280.
Poisk vzaimosvjazej mezhdu parametrami kinetiki kislotnogo gemoliza jeritrocitov i funkcional'nym sostojaniem organizma / I.L.Golenda [i dr.] // Fiziologija cheloveka. – 1996. – V. 4 (22). – S. 130-136.
Ivanov I.T. Kinetika kislotnogo gemoliza v izotonicheskoj srede saharozy. / I.T. Ivanov, Ju.D. Danailova. // Biofizika. – 1991. – V. 36 (5). – S. 845-849.
Zavodnik I.B. Kislotnyj lizis jeritrocitov cheloveka. / I.B. Zavodnik, T.P. Pileckaja // Biofizika. – 1997. – V. 5 (42). – S. 1106-1112.
Pezistentnost' k kislotnomu gemolizu jeritrocitov krolej pri jeksperimental'nom saharnom diabete i primenenii saharosnizhajushhih sredstv / T.A. Shatalova [i dr.] // Biofizicnij visnik. – 2013. – V. 2 (30). – S. 35-41.
Ivanov I.T. Sravnenie mehanizmov kislotnogo i shhelochnogo gemoliza jeritrocitov cheloveka / I. T. Ivanov // Biofizika. – 2001. – V. 2 (46). – S. 281–290.
Terskov I.A. Metod himicheskih (kislotnyh) jeritrogramm / I.A. Terskov, I.I. Gitel'zon // Biofizika. – 1957. – V. 2 (2). – S. 259–266.
Gitel'zon I.I. Sostav krasnoj krovi v norme i patologii / I.I. Gitel'zon. – Tomsk, 1960. – S. 187.
Vochegorskij I.A. Antioksidanty pri jeksperimental'nom saharnom diabete / I.A. Vochegorskij, L.M. Rassohina, I.Ju. Miroshnichenko // Probl. jendokrinologii. – 2008. – V. 54 (5). – S. 43–49.
Balabolkin M.I. Rol' okislitel'nogo stressa v patogeneze sosudistyh oslozhnenij saharnogo diabeta i primenenie vitaminov i mikrojelementov dlja ih lechenija i profilaktiki / M.I. Balabolkin // Klinicheskaja jendokrinologija. – 2006. – V. 6. – S. 1–7.
В.О. Китаева, Ф.А. Гершкорон // Научный медицинский вестник ЮГРЫ. – 2014. – Вып. 1-2 (5-6). – С. 79–81. / Kitaeva V.O. Opredelenie kislotnoj rezistentnosti jeritrocitov i urovnja gormona stress-reakcii jestradiola u bol'nyh ishemicheskoj bolezn'ju serdca. / V.O. Kitaeva, F.A. Gershkoron // Nauchnyj medicinskij vestnik JuGRY. – 2014. – V. 1-2 (5-6). – S. 79–81.
Galenok V.A. Gemoreologija pri narushenijah uglevodnogo obmena / V.A. Galenok, E.V. Gostinskaja, V.E. Dikker. – Novosibirsk, 1987. – S. 86.
Mal'ceva I.V. Harakteristika rezistentnosti jeritrocitov u kardiohirurgicheskih bol'nyh s razlichnoj stepen'ju vyrazhennosti postperfuzionnogo gemoliza / I.V.Mal'ceva // Bjulleten' sibirskoj mediciny. – 2013. – T. 12, V. 1. – S. 69–74.
Rukovodstvo po gematologii: v 3 t. / Рod red. A.I. Vorob'eva: 3-e izd., pererab. i dop. - M.: N'judiamed, 2005. - 416 s.
Castellino R.A. Computer aided detection (CAD): an overview / R.A. Castellino // Cancer Imaging. – 2005. - V 5 (1). – Р. 17–19.
Pokrajac L. Oligomerization and hemolytic properties of the C-terminal domain of pyolysin, a cholesterol-dependent cytolysin / L. Pokrajac. J.R. Harris, N. Sarraf, M. Palmer // Biochem. Cell Biol. – 2013. – V. 91, N. 2. – P. 59-66.
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