Immunomorphological features of women's skin with androgenetic alopecia in the treatment of plasma enriched with platelets in combination with topical minoxidil 2 % lotion

DOI: https://doi.org/10.26565/2313-6693-2021-43-09
Keywords: androgenetic alopecia, platelet-rich plasma, minoxidil, immunohistochemical examination of skin biopsies

Abstract

Introduction. The skin biopsies of 30 patients with androgenetic alopecia before and after treatment with platelet-rich plasma in combination with 2 % minoxidil were examined by immunohistochemistry. Goal. The study looked at immunohistochemical changes in the scalp biopsies of patients with stage I-II androgenetic alopecia according to the Ludwig scale and to identify possible changes after injection of platelet-rich plasma in combination with topical 2 % minoxidil lotion. Materials and methods. Skin biopsies of 30 patients with androgenetic alopecia were examined using an immunohistochemical study before and after treatment, which lasted 3 months and included 3 sessions of PRP (once per month) and topical application of minoxidil 2 % lotion twice a day. Results. It was found that androgenetic alorecia is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of germ polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of collagen IV. Injections of platelet-rich plasma in combination with topical administration of 2 % minoxidil as a treatment for androgenetic alopecia lead to normalization of immunohistochemical parameters of the skin, which indicates the possibility of using this combination for long-term therapeutic effect. Conclusions. This study complemented the understanding of the pathogenesis of AGA and serves as a basis for improving treatment regimens for this pathology. However, additional studies are needed to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.

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Author Biographies

Olena Salyenkova, V. N. Karazin Kharkiv National University School of Medicine

Assistant of the Department of infectious diseases and clinical immunology, V. N. Karazin Kharkiv National University, Medical Faculty, 6, Svobody sq., Kharkiv, Ukraine, 61022

Svitlana Danyliuk, Kharkov Medical Academy of Postgraduate Education

Associate Professor of the Department of Pathological Anatomy and Histology of the Kharkov Medical Academy of Postgraduate Education, 58, Amosova st., Kharkov, Ukraine, 61176

Yuliya Ovcharenko, V. N. Karazin Kharkiv National University School of Medicine

PhD, Professor, Department of infectious diseases and clinical immunology, V. N. Karazin Kharkiv National University, Medical Faculty, 6, Svobody sq., Kharkiv, Ukraine, 61022

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Published
2021-12-01
How to Cite
Salyenkova, O., Danyliuk, S., & Ovcharenko, Y. (2021). Immunomorphological features of women’s skin with androgenetic alopecia in the treatment of plasma enriched with platelets in combination with topical minoxidil 2 % lotion. The Journal of V. N. Karazin Kharkiv National University, Series "Medicine", (43). https://doi.org/10.26565/2313-6693-2021-43-09
Issue
No. 43 (2021): The Journal of V. N. Karazin Kharkiv National University. Series "Medicine"
Section
Clinical research

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