The Journal of V. N. Karazin Kharkiv National University, series "Medicine" <p>Journal contains the following sections: Healthcare Organization; Fundamental Research; Clinical researches; Reviews; Clinical Practice; Lectures; Medical Law; Ecology and Health.</p> <p><em><strong>The Journal of V. N. Karazin Kharkiv National University, series "Medicine"&nbsp; Teaching is included in the list of professional scientific publications recommended for publication of the main scientific results of dissertations by the decision of the Ministry of Education and Science of Ukraine (<a href=";issnSearch=2313-6693%20&amp;">№ 886 от 02.07.2020</a>).</strong></em></p> V. N. Karazin Kharkiv National University en-US The Journal of V. N. Karazin Kharkiv National University, series "Medicine" 2313-6693 <p>The Journal of V. N. Karazin Kharkiv National University, series Medicine has following copyright terms:</p><ol><li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.</li><li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work, with an acknowledgement of its initial publication in this journal.</li><li>Authors are permitted and encouraged to post their work online prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.</li></ol> FULL VERSION OF THE ISSUE FULL VERSION OF THE ISSUE No. 1 NN NN ##submission.copyrightStatement## 2015-07-04 2015-07-04 1 1 114 PERMEABILITY OF BLOOD BRAIN BARRIER FOR CARDIOTROPIC SUBSTANCES UNDER HYPOTHERMIA AND HYPERTENSION <span>It has been shown that under hypothermia conditions the interactions between central and autonomous contours of cardiac activity regulation can be significantly changed, therefore the probability of paradoxical reactions of an organism to application of cardiotropic preparations increases. The change of a sign of chronotropic effect of cathecholamines (CA) and acethylcholine (AC) under hypothermia in a great extent is substantiated by an increase of blood brain barrier (BBB) permeability, that should be taken for consideration in practical medicine. Besides therapeutic cooling regimens contribute to a recovery of impaired rhythmicity of functioning of nerve and cardiovascular systems, in particular under conditions of emotional stress, that is one main causes of human disease appearing.</span> V.G. Babijchuk ##submission.copyrightStatement## 1 16 21 THE POLYVINYLPYRROLIDONE CONTENT CONTROL IN DRUGS AND BIOLIQUIDS <span>The spectrophotometric method of determination of polyvinylpyrrolidone (PVP) with using of organic dyes brilliant yellow (BY) and bromophenol blue (BPB) is elaborated for the content control of PVP in medicinal object. The method has been tasted for determination of PVP in Haemodesum - N and also in urine. The error of determination is less than 4%.</span> F.A. Chmilenko Yu.S. Sapa T.S. Chmilenko M.V. Kharun ##submission.copyrightStatement## 1 22 24 ISCHEMIA EFFECT ON ATPASE ACTIVITY OF HEART MITOCHONDRIA IN YOUNG AND OLD RATS <span>ATPase activity alterations in rats of different age groups during the total myocardial ischemia were studied. It was determined that only mitochondrial oligomycin-sensitive ATPase was sensitive to effect of the total myocardial ischemia. The fact that addition of magnesium ions in the incubation medium eliminated ischemic effect in 3-months old rats and weakened it in 24-months old rats pointed to important role of magnesium ions in structure-functional reconstruction of mitochondria under myocardial ischemia. Young 3months old rats were established to be more tolerant to myocardial ischemic damage thanold animals.</span> V.N. Dzuba Yu.V. Nikitchenko T.A. Shmonina ##submission.copyrightStatement## 1 25 29 EEG PECULIARTITIES AND KINDLED SEIZURES UNDER CONDITION OF TRANSCRANIAL MAGNETIC STIMULATION <table width="550" border="0" cellspacing="0" cellpadding="3"><tbody><tr><td align="left" valign="middle" width="504"><p align="justify">Transcranial magnetic stimulation (TMS) at low frequency (2/s for 10 s, 0.1 Tl at the heightof the impulse peak) induced an increase in delta bandwidth power and a marked reduction intheta and alpha rhythms in the basal EEG in rats kindled via amygdalar electrical stimulation(ES). A marked reduction in beta and gamma bandwidth power was also seen. All effects wereseen within half an hour of TMS and were brain structure-dependent. Amygdalar ES did notinduce generalized clonic-tonic fits when applied to kindled rats after TMS. The duration ofgeneralized epileptoform activity was also shorter after TMS. A reduction in theta, beta, andgamma rhythms and an increase in delta rhythm at the moment of cessation of epileptiform discharge (ED) (last 16 s of discharge) in TMS-kindled rats were observed. Thus, a suppressiveantiseizure effect can be seen when relatively low intensity TMS is used, and the effects might be mediated through delta rhythmogenesis activation.</p></td></tr></tbody></table> L.S. Godlevsky A.A. Shandra A.I. Brusentsov E.M. Barnyak A.M. Mazko A.V. Mandel A.V. Zhylinskaya A.A. Oleynik ##submission.copyrightStatement## 1 30 35 THE VARIETY OF FACTORS THAT INFLUENCE THE BIOMECHANICAL PROPERTIES OF THE LIMB SKIN DURING LENGTHENING <span>The aim of this work is to analyze the influence of some factors on biomechanical behavior of the human skin in natural growth and in graduated lengthening with application of the Ilizarov fixator. 45 healthy individuals of various age and 104 patients suffering from congenital shortening of one lower limb or osteomyelitic sequelae have been examined. The limb discrepancy was eliminated by mono- and bilocal osteosynthesis with application of the Ilizarov fixator. The amount of lengthening comprised 3 - 14 cm, its relative value achieved 6 - 30%. Distraction started in 5 days following fixator placement. Biomechanical parametres were determined with the help of the device designed by A.A. Utenkin. The method allows for studying skin deformation properties - shift stiffness (S, kg/mm2) by minimal in time loading but constant in value moment, as well as for calculation of the amount of relative deformation (stretching,., %). The work reports on the effect of different factors – structural and age ones, conditions of reparative lengthening and processes of regeneration – on the mechanical behavior of the limb skin in shortening of different etiology and gradual traction for equalizing limb length. There is a number of factors having influence on the skin biomechanics of the human extremity: 1 structural peculiarities of the collagen bundles and elastic fibres in the papillary and reticular dermis layers (collagenous fibres make up 98% of the connective dermis tissue and provide its high stiffness). The conformation principle of the collagenous and elastic fibrillas and fibres is their spiral character. 2 - the type of the collagen viscosity in the dermis, especially in its deep layers; 3 - age factor; 4 - an amount and etiology of limb shortening in patients with the locomotor system orthopaedic pathology; 5 - the method of limb shape modeling (lengthening and deformity correction) in the conditions of the guided transosseous osteosynthesis (mono- and bilocal osteosynthesis); 6 - skin regeneration (Ilizarov effect) as a consequence of the formogenetic effect appearing in the conditions of tension stress.</span> L.A. Grebeniuk A.V. Popkov V.A. Shchurov ##submission.copyrightStatement## 1 36 40 MATHEMATICAL MODEL OF THE UTERINE CONTRACTIONS IN LABOR <span>The article presents a mathematical model of the uterine labor activity as continuous oscillations within the limits ot the system of uterus-fetus-amnion.</span> І.V. Lakhno ##submission.copyrightStatement## 1 41 43 THE LEVEL OF TRYPTOPHAN AND SEROTONIN IN THE CONVULSIVE READINESS CONDITIONS OF CEREBRUM <span>It was determined that the serotonin level in hypothalamus, brain stem, cerebellum and brain hemispheres lowers in rats with high audiogenic spasmodic activity as compared to rats with low excitability level, and the serotonin content in rats decreases in cerebellum and hypothalamus.</span> E.G. Maksimenko V.N. Savchenko ##submission.copyrightStatement## 1 44 47 BLOOD LEVELS OF SUBSTANCE P AND BETA-ENDORFINS AND EXPERIMENTAL MYOCARDIAL INFARCTION CICATRISATION <span>The experiment over 30 dogs with myocardial infarction (MI) model shows that the blood dynamics ofmaintenance and balance of substance P (SP) and beta-endorphins (BE) are in compliance with MI cicatrisation outcomes. For the cicatrisation without compliances the CP predominance in the acute period and BEpredominance in the sub-acute period is typical. The CP predominance during the whole experiment appearsto be determinative for the complicated MI cicatrisation. Monitoring of the CP and BE levels can be considered as one of timely methods of diagnosing and healing of the complicated IM cicatrisation.</span> A.A. Moybenko V.N. Sokrut I.R. Shvirenko N.I. Yabluchansky ##submission.copyrightStatement## 1 48 51 INTERRELATION BETWEEN CONVULSIVE READINESS AND THE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN THE CEREBRUM OF ANIMALS <span>As a result of the conducted experiments it was determined that the noradrenaline (NA) level in the hypothalamus and brain stem decreased in rats with high genetically determined spasmodic readiness as compared to the rats of other groups, and in hemispheres, brain stem and cerebellum the dophamine (DA) content rose significantly. The decrease of tyrosinelevel in the brain stem and cerebellum was statistically proved. It gives grounds to supposethat increase in spasmodic readiness is correlated with processes influencing both catecholamine complexes and serotonine.</span> V.N. Savchenko E.G. Maksimenko ##submission.copyrightStatement## 1 56 62 THE MUTAGENIC TESTS OF NEW AUXILARY FARMACEUTICAL SUBSTANCIES FOR MAMMALIANS <span>The potential mutagenic effects of new auxilary farmaceutical substancies (emulgators) was investigated. Under per os injection the substancies not induced the chromosomal aberrations in the narrow cells and dominant lethal mutations in male gametes of mouse. The results are discussed in connection to using at investigated substancies in pharmacology.</span> N.G. Strygelchyk ##submission.copyrightStatement## 1 63 67 COMPARATIVE STUDY OF THE SMALLER INTESTINE VILLUS EPITHELIUM UNDER EXPERIMENTAL PERITONITIS AND INTESTINAL OBSTRUCTION <span>A comparative study of the smaller intestine villus epithelium ultrastructure under experimental peritonitis and intestinal obstruction was carried-out. It was shown that ultrastructural rearrangements of the smaller intestine epithelium start after 6 hours of an inflammatory process. Destructive changes in cells under intestinal obstruction were more pronounced than under peritonitis.</span> T.I. Tamm A.Y. Barduk T.P. Govorucha N.V. Repin ##submission.copyrightStatement## 1 68 71 THE ANALYSIS OF VIABILITY EVALUATION METHOD FOR MICROORGANISM CELLULAR FORMS AFTER CRYOPRESERVATION <span>The review of existing evaluation methods of viability of microorganisms is presented. The feasibility to apply these methods for the estimation of viability of microorganisms after cryopreservation was analysed.</span> I. P. Vysekantsev N.G. Kadnikova V.F. Martsenyuk ##submission.copyrightStatement## 1 77 80 RHEABILITATION RESULTS OF PATIENTS WITH ACCIDENT RADIATION INJURIES <span>The purpose of the work based upon the treatment results of 29 patients with radiation injuries received in accidents are to determine optimum his treatment tactics. Special attention was focused on surgical rehabilitation and mistakes and inaccuracies, made during its carrying out. The calculation of the received dozes was carried out of the basis of the calculated simulation method of an accident in consideration of the source’s activity, geometry of the exposure and time of contact with it. The observation of the patient under considerations enables us to assert that in the acute period of the radiation injury (prior to 3 months after the accident) the reconstructive surgical treatment is expedient only in conditions of x-ray overexposure, with only surface injure of tissues, especially when there is their inflammatory response. For better remote results of treatment surgical rehabilitation of the consequences of a radiation trauma, including determination of the coretraction scope and the type of an operation, should be postponed for 6 months, which will enable to do it more precisely.</span> V.A. Moroz V.P. Starenkiy ##submission.copyrightStatement## 1 52 55 MORPHOMETRICAL CHANGES OF DUPLEX KIDNEYS IN CHILDREN IN DIFFERENT DEGREE OF URODYNAMICS INFRINGEMENT AND WITHOUT URODYNAMICS INFRINGEMENT <span>Morphometrical comparison of duplex kidney and ureter structural elements in children was done taking in account the gravity of the pathological process and methods of treatment. Morphological objects giving the possibility to prognose the method of surgical treatment and it’s outcome depending on the quality and quantity of changes of the duplex kidney structure were determined. The principal difference of quantitative changes of structural elements of a duplex kidney without the violation of urodynamics in moderate violation of the outflow of urine and also in a steadfast loss of the function because of the violation of urodynamics of a grave degree was confirmed.</span> G.I. Taran ##submission.copyrightStatement## 1 72 76 TREATMENT OF ACUTE PURULENT CHOLANGITIS WITH NASOBILIAR DRAINAGE <span>The results of treatment the 34 patients with the acute purulent cholangitis was analysed. There were 9 men (26,4%) and 25 women (73,6%) in the age for 43 to 87. On the entrance those patients to the clinic their general condition were heavy, but some of them had middle weight condition. The possibility violation of the gall-ducts on the level of common bile ductwere conditioned with: the big stones have 18 patients (52,9%), which defied to endoscopiccholedocholithotraction; strictures of the ducts have 8 patients (24,7%); combination ofcholedocholithiasis with the strictures have 5 patients (14,7%); combination of the choledocholithiasis with parapapillar diverticulum have 3 patients (8,8%). The diagnostic program conclude of the clinical and laboratories methods investigations, ultrasound scanning, endofibroscopic investigation the top past of the gastro-intestinal tract, endoscopic retrograde cholangiopancreatography, cholangiomanometry, bacteriological cheek up of the bile. The treatment program included: the standard infussional therapy of acute cholangitis and the obstructive jaundice; the endoscopic sphincterotomy with following nasobiliar drainage; thelandmark endocholangial leading the complex antibacterial medicine Levosin. As the resultsof the treatment all patients with acute obstructive purulent cholangitis in all cases weremixed in the period of 4-9 days after the treatment starting, with were definite by the clinicalflowing and verified by the dates of laboratorical cheek up methods. All of these allowed tofulfil the radical surgical intervention on the common bile duct with the least risk for the patient. There was no lethal outcome.</span> Yu.B. Zakharchenko ##submission.copyrightStatement## 1 81 84 SHORT – TERM HRV PARAMETERS STABILITY IN ATRIAL FIBRILLATION PATIENTS <span>The object under study was time and frequency domain HRV parameters stability in 15atrial fibrillation patients. The mean age of patients was 61±8,7. HVR was evaluated using “Cardiolab 2000”. 30 minutes ECG intervals were recorded and subsequently divided into six equal 5 minutes intervals. Time domain (mRR, sdRR, pNN50, HRVTi) and frequency domain (TP, VLF, LF and HF) parameters of heart rate were evaluated. The time stability of the HRV parameters wasestimated basing on the variation coefficient value (C). Three classes of HVR parametersstability were determined: class 1 – high stability (C&lt;0,1), class 2 – stability of middle degree(C=0,1-0,2), 3 class – low stability (C&gt;0,3). Class 1 included mRR, sdRR and pNN50; class2 – HRVTi, TP, VLF and HF; LF was refered to class 3. Thus the results indicated thatatrial fibrillation is characterized by a time rigidity. Time domain HVR parameters exhibitmore and frequency domain less pronounced stability degrees.</span> L.A. Martimyanova N.V. Makienko ##submission.copyrightStatement## 1 85 90 HEART RATE VARIABILITY IN THE PATIENTS WITH CORONARY ARTERY DISEASE DURING SESSION OF HYPERBARIC OXYGENATION THERAPY <span>Prior trials detected benefits from hyperbaric oxygenation therapy (HBOT) for patients with coronary artery diseases (CAD) and acute myocardial infarction. The aim of this study was to assess the changes in ANS in patients with CAD during session of HBOT by using the technique of HRV. The patients with CAD were subdivided into three groups in dependence of atmosphere pressure and contents of oxygen. We measured the total power (TP), low frequency component (LF) reflecting sympathetic and high frequency (HF) reflecting vagal activity, autonomic balance (LF/HF ratio) and LF and HF in normalized units (LFn, HFn). We observed significant increase under HBOT of TP, decreased LF/HF ratio because of changing in both branch of AHS and parasympathetic activity increased more significant than decreased sympathetic activity. In our point of view the changes in ANS depend of proportionally from oxygen tension in tissues coursed by HBOT. Our preliminary results showed that measurement of HRV is helpful in monitoring not only the circulation but also the common status of patients during the sessions of HBOT and it will be possible in the future to modulate the protocols of HBOT individually for certain patient. Increasing of vagal activity and total power with decreasing of sympathetic tone during of HBOT is the evidence of "relaxation" of circulation and ANS which less stressed by monitoring the body oxygenation. Adjunctive HBOT can improve prognosis in patients with CAD in various ways: the positive modifying of HRV is a sign of favorable changing in ANS; increasing of cardiac electrical stability; prevention of left ventricular dysfunction, progressing in Killip class and ventricular ectopic activity and presence of late potentials; restores of sinus nodal cells to neural modulations.</span> A.V. Stepanov ##submission.copyrightStatement## 1 91 96 USE PRIMOLUT-NOR FOR TREATMENT ENDOCRINE STERILITY AT THE WOMEN WITH HYPERPLASIA OF ENDOMETRIUM <span>The complex inspection and treatment 90 patients with endocrine sterility with presencehyperplasia of endometrium is carried out which were divided into 2 clinical groups till 45persons in each. For an induction ovulation the patients of both groups nominated klostilbegit on a standard technique, but in the second group have carried out preliminary correctionof processes hyperplasia in endometrium in by purpose primolut-nor. The offered methodof preparation of the women with endocrine sterility in a combinationwith with hyperplasia of endometrium promoted increase of percent( of approach of pregnancy from 33,6 % up to 62,2 % and decrease of spontaneous abortions in term up to 12 week from 37,5 % up to 14,2%.</span> A.M. Feskov ##submission.copyrightStatement## 1 97 100 VIDEOLAPAROSCOPIC CHOLECYSTECTOMY IN PATIENTS SUBJECTED TO OPERATIONS ON ORGANS OF THE ABDOMINAL CAVITY IN THE EARLIER PERIOD <span>For the period from 1994 year 1486 patients with chronic and acute calculosus cholecystitis aged from 18 to 78 years old were operated in the hospital. 312 patients of them were subjected to operations on organs of the abdominal cavity in the earlier period. Peculiarities of the operative treatment in this group of patients are elucidated in the article, particularly carrying out of the videolaparoscopic cholecystectomy. Au thors based possibility and expedientness of fulfilment of videolaparoscopic cholecystectomy in patients subjected to operations on organs of the abdominal cavity earlier.</span> E.D. Khvorostov A.I. Tsivenko S.A. Bychkov ##submission.copyrightStatement## 1 101 103 ULTRASOUND AND COMPUTER TOMOGRAPHY IN STAGING OF PANCREATIC CARCINOMA IN PATIENT WITH CHRONIC PANCREATITIS <span>The aim of present work was to determine the possibility of ultrasound and computed tomography in the diagnosis and staging of pancreatic carcinoma in combination with chronic pancreatitis. Rel i a bl e cr i t er i a of pa n cr eatic tumor ar e th e followi n g in di r ect si gn s: tumor exten sion to ad ja cen t st r u ct u r es, lymph n ode in vol vemen t an d di st an t metast ases. On ly in ter ven tion a l pr ocedur e with mor ph ologi ca l assessm en t ar e va luable meth ods in di ag n osi s of p a n cr eatic ca r cin om a in con n ection with ch r on i c pa n cr eatitis.</span> I.P. Vakulenko N.V. Momot E.A. Savchenko ##submission.copyrightStatement## 1 104 107 COMBINATION OF METAPROLOL AND CONJUNCTIVE FORM OF ENALAPRIL MALEAT AND HYDROCHLOTHIAZIDE IN SMALLER DOSES ALLOWS ACHIEVINGTHERAPEUTICAL EFFECT IN HYPERTENSION PATIENTS BETTER MODERATE PHARMACOARTERIAL <span>The object of the present study was to evaluate the clinical effectiveness of the beta-blockers and ACE- inhibitors treatment of hypertension patients. The group of patients included 72 persons aged from 27 to 68 (47±6,97) with a moderate hypertension remaining for 7,4±5,2 years. All patients were divided into 3 groups. The first group (27 patients) received monotherapy of the conjunctive from of enalapril and hydrochlorothiazide (2 tablets twice a day), the second group (10 patient) was getting metaprolol (100 mg/daytwice a day). The third group of patients received both medications simultaneously once a day. Heart ratevariability was obtained by using “Cardiolab 2000“ device. The total power of spectrum (TP, ms2) wasevaluated as a measure of the total neuro-humoral regulation. Power of spectrum in the very low (VLF, ms2), low (LF, ms2) and high (HF, ms2) frequency areas reflected the humoral, sympathetic and parasympatheticinfluences correspondently. The low and high frequency areas ratio was obtained as an index of the sympatho-vagal balance (LF\HF). According to the ТР value obtained in the baseline the patients were divided into the sub-groups with a satisfactory (ТР&gt;1000 мс2) and low (ТР&lt;1000 мс2) power of the total regulation. The treatment effectiveness in each group was evaluated by comparing its results in sub-groups withdifferent basal regulation status. The reliability of the discrepancies was obtained by using the Studentcriterion for 95% confiding interval. Positive influence of the ACE inhibitors and beta-blockers was observednot only in the arterial pressure dynamics but also in the neuro-humoral regulation status. The optimisinginfluence on the regulation systems became apparent not only as a growth of the total regulation but also asnormalization of its branch balance (first of all the sympatho-vagal balance). The combined therapy providedmore pronounced clinical result with smaller doses then the regular therapy. It also made the treatment lessexpensive.</span> N.I. Yabluchansky E.P. Kamenskaya ##submission.copyrightStatement## 1 108 112 USUAL MEDICAL DEPARTMENT? NO, MUCH BETTER! (HISTORICAL REVIEW) <span>The medical education activity of the Karazin Kharkiv National University was renewed with the Ukraine becoming an independent state. Historical roots and the actuality of the University medical education is the topic of the article. The main idea is that medical education in the Ukraine should turn toward the educational sphere. In this way the conflict of the interests caused by concentration in one place both the medical education and health care will disappear. This is going to be profit for everyone and for ordinary citizen in the first place. This means that whole country will be benefited.</span> M.I. Yabluchansky ##submission.copyrightStatement## 1 6 11 GYNECOLOGICAL ENDOCRINOLOGY: A HISTORICAL REVIEW (lecture for future obstetricians and gynecologists) <span>A review of the most important discoveries of female reproductive anatomy and physiology on theway to current status of gynecological endocrinology.</span> O.V. Grischenko І.V. Lakhno ##submission.copyrightStatement## 1 12 15