FEATURES OF CYTOKINE STATUS IN PATIENTS WITH CHRONIC EBV-INFECTIONS
Abstract
Infections caused by EBV are the most common and occupy an important place in the structure of herpes aetiology diseases. The purpose of this work was to study the characteristics of the cytokine status in patients with chronic EBV infection, depending on the level of viral replication. We examined 78 patients with chronic EBV infection, the main clinical manifestations of which were various immunopathological and immunodeficiency states: Group I – with low, Group II – with medium, Group III - with a high degree of viral replication. The Tiff method was used using the Vector-Best reagent kits (Novosibirsk, Russia) to study the cytokine profile in the serum of patients with EBV infection. The determination of alpha and gamma fractions of serum interferon was carried out using the ELISA method by means of the ProCon IF2 plus reagent kit manufactured by Protein Contour LLC (St. Petersburg, Russia). As a result of a study of the cytokine status in patients with chronic EBV infection, it was found that in all three groups there was a significant increase in both pro-inflammatory (IL-1β, IL-6, TNF-α) and antiinflammatory cytokines (IL-10, IL 4, TGFβ1). However, anti-inflammatory cytokinemia was more compensated in group I patients compared with patients in groups II and III. A decrease in IFN-α and IFN-γ was detected in all patients with chronic EBV infection while studying the interferon status. A correlation was found between the level of viral replication and a decrease in the level of IFN-α and IFN-γ. The identified features of the cytokine status in patients with chronic EBV infection can be used to optimize therapy and help develop a differentiated approach to the immunocorrection of these patients, depending on the level of viral replication.
Downloads
References
Kliniko-laboratorni osobly`vosti perebigu infekcijnogo mononukleozu u dorosly`x / P. P. Kish., G. M. Koval`, V. O. Petrov [ta in.] // Naukovy`j visny`k Uzhgorods`kogo universy`tetu. – 2013. – T.
– No. 47. – S. 139–144. 2. Kan N. Yu. Znachenie persistiruyuschey gerpesvirusnoy infektsii v formirovanii vtorichnogo immunodefitsita u chasto boleyuschih detey // Detskie infektsii: Nauch.-prakt. zhurn. assots. pediatrovinfektsionistov. – M.: OOO «Diavaks», 2008. – No. 2. – S. 66−67.
Lan K., Luo M. H. Herpesviruses: epidemiology, pathogenesis, and interventions. – 2017.
Smatti M. K. et al. Epstein–Barr virus epidemiology, serology, and genetic variability of LMP-1 oncogene among healthy population: an update // Frontiers in oncology. – 2018. – Т. 8.
Bodnar V. A. Udoskonalennya diagnosty`ky` xronichny`x form infekciyi, zumovlenoyi virusom Epshtejna–Barr // Problemy` ekologiyi i medy`cy`ny`. – 2013. – T.2. – No. 5–6. – S. 9–15.
Dambaeva S. V., Mazurov D. V., Klimova S. V. Otsenka osnovnyih parametrov immunnoy sistemyi s pomoschyu protochnoy lazernoy tsitometrii // Allergiya i immunologiya. – 2002. – T.3. – No. 3. – S. 371−379.
Sorokina O. G. Doslidzhennya klity`nnogo imunitetu u xvory`x na xronichnu VEB-infekciyu // Imunologiya ta alergologiya: nauka i prakty`ka. – 2018. – No. 3. – S. 65−71.
Kondo S. et al. Expression of interferon regulatory factor 7 correlates with the expression of Epstein–Barr Virus latent membrane protein 1 and cervical lymph node metastasis in nasopharyngeal cancer // Pathology international. – 2017. – Т. 67. – No. 9. – p. 461–466.
Jud A. et al. Tonsillar CD56brightNKG2A+ NK cells restrict primary Epstein-Barr virus infection in B cells via IFN-γ // Oncotarget. – 2017. – Т. 8. – No. 4. – p. 6130.
Ohga S. et al. Dominant expression of interleukin‐10 and transforming growth factor‐β genes in activated T‐cells of chronic active epstein–barr virus infection // Journal of medical virology. – 2004. – Т. 74. – No. 3. – p. 449–458.
Lünemann J. D. et al. EBNA1-specific T cells from patients with multiple sclerosis cross react with myelin antigens and co-produce IFN-γ and IL-2 //Journal of Experimental Medicine. – 2008. – Т. 205. – No. 8. – p. 1763-1773.
Nagu T. et al. Strong anti-Epstein Barr virus (EBV) or cytomegalovirus (CMV) cellular immune responses predict survival and a favourable response to anti-tuberculosis therapy // International Journal of Infectious Diseases. – 2017. – Т. 56. – p. 136–139.
Li Y. et al. Haemophagocytic lymphohistiocytosis in inflammatory bowel disease with virus infection // Przeglad gastroenterologiczny. – 2015. – Т. 10. – No. 2. – p. 78.
Chinen T. et al. An essential role for the IL-2 receptor in T reg cell function // Nature immunology. – 2016. – Т. 17. – No. 11. – p. 1322.
Jiang T., Zhou C., Ren S. Role of IL-2 in cancer immunotherapy //Oncoimmunology. – 2016. – Т. 5. – No. 6. – p. e1163462.
Ben-Ami E., Miller A., Berrih-Aknin S. T cells from autoimmune patients display reduced sensitivity to immunoregulation by mesenchymal stem cells: role of IL-2 // Autoimmunity reviews. – 2014. – Т. 13. – No. 2. – p. 187–196.
Gideon H. P. et al. Variability in tuberculosis granuloma T cell responses exists, but a balance of pro-and anti-inflammatory cytokines is associated with sterilization // PLoS pathogens. – 2015. – Т. 11. – No. 1. – p. e1004603.
Wojdasiewicz P., Poniatowski Ł. A., Szukiewicz D. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis // Mediators of inflammation. – 2014. – Т. 2014.
Neurath M. F. Cytokines in inflammatory bowel disease // Nature Reviews Immunology. – 2014. – Т. 14. – No. 5. – p. 329.
Frangogiannis N. G. The inflammatory response in myocardial injury, repair, and remodelling // Nature Reviews Cardiology. – 2014. – Т. 11. – No. 5. – p. 255.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The Journal of V. N. Karazin Kharkiv National University, series Medicine has following copyright terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work, with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
