Peculiarities of immunore resistance changes in the experiment of induced peritonitis in animals of different ages

  • Marina Ivanenko PhD in Biology, s.r.f. Laboratory of Radiation Immunology, SI «Grigoriev Institute for Medical Radiology of National Academy of Medical Sciences of Ukraine» https://orcid.org/0000-0002-0466-6008
  • Olena Klimova Doctor of Biology, full professor, Head of diagnostic laboratory with immunoassay and immunofluorescence analysis, V. T. Zaytsev Institute of General and Urgent Surgery of National Academy of Medical Sciences of Ukraine
  • Serhii Sherstiuk Doctor of Medicine, full professor, Head of the Department of Human Anatomy, V. N. Karazin Kharkiv National University https://orcid.org/0000-0001-6062-7962
  • Svitlana Nakonechna PhD in Biology, associate professor of the Department of Human Anatomy, V. N. Karazin Kharkiv National University
Keywords: blood serum, immunoglobulin A, circulating immune complexes, phagocytosis, resistance factors

Abstract

Introduction. The protection of the body against external and internal antigenic factors is executed with the help of the primary cellular and secondary resistance links. Excessive activation of adaptation reactions leads to the formation of various pathologies of inflammatory nature. Changes in the immune responses occur at all ontogenesis stages. In the present study, we conduct the experiment of induced peritonitis in animals of different ages in order to investigate more accurately adaptive responses of the immune system during inflammation.

Objectives. The aim of our research was to study changes in the indicators of adaptive humoral immunity, levels of immunoglobulin A and circulating immune complexes, phagocytic activity of neutrophils and the disruption of enzymes activity, which provide the phagocyte function in the NST test on the model of induced peritonitis in animals of different ages.

Materials and methods. The studies were performed on 200 white male rats. They were divided into a control group and the experimental rats, 3- and 22-month-old ones. Acute inflammation and dysbiosis in the small intestine were caused by intraperitoneal injection of lipopolysaccharide obtained from Escherichia coli strain. The material for the study was serum and blood elements of experimental animals.

Results. The levels of immunoglobulin A in the blood serum of 3 and 22-month-old rats with the inflammation model were reduced in comparison with this index in control group animals. The content of the CIC in the rats blood serum of both age groups was significantly higher in comparison with the control group. All the studied indices of neutrophils phagocytic activity in the 22-month-old animals with the inflammation model were lower than in the control rats of this age. In the 3-month-old rats with the inflammation model, the index of phagocytosis completeness was significantly lower in comparison with the control group. The reduction in the reserve capacity of phagocytic cells was higher in the 22-month-old animals. An increase in the neutrophils metabolic activity and a decrease in their metabolic reserve in 3 and 22-month-old rats with the inflammation model were revealed in comparison with the parameters of the control groups.

Conclusions. The results of the study indicate presence of violation of the primary cellular and  secondary humoral immunity during the aging of the body and decrease in the adaptive responses of the immune system during inflammation due to an increase in antigenic effects.

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Author Biographies

Marina Ivanenko, PhD in Biology, s.r.f. Laboratory of Radiation Immunology, SI «Grigoriev Institute for Medical Radiology of National Academy of Medical Sciences of Ukraine»

Pushkinskaya str., 82, Kharkiv, 61024, Ukraine

Olena Klimova, Doctor of Biology, full professor, Head of diagnostic laboratory with immunoassay and immunofluorescence analysis, V. T. Zaytsev Institute of General and Urgent Surgery of National Academy of Medical Sciences of Ukraine

entrance Balakirev, 1, Kharkiv, 61103, Ukraine

Serhii Sherstiuk, Doctor of Medicine, full professor, Head of the Department of Human Anatomy, V. N. Karazin Kharkiv National University

6, Svobody Sq., Kharkiv, 61022, Ukraine

Svitlana Nakonechna, PhD in Biology, associate professor of the Department of Human Anatomy, V. N. Karazin Kharkiv National University

6 Svobody Sq., Kharkiv, 61022, Ukraine

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Published
2019-06-13
How to Cite
Ivanenko, M., Klimova, O., Sherstiuk, S., & Nakonechna, S. (2019). Peculiarities of immunore resistance changes in the experiment of induced peritonitis in animals of different ages. The Journal of V. N. Karazin Kharkiv National University, Series "Medicine", (37), 5-11. https://doi.org/10.26565/2313-6693-2019-37-01