Peculiarities of immunore resistance changes in the experiment of induced peritonitis in animals of different ages
Introduction. The protection of the body against external and internal antigenic factors is executed with the help of the primary cellular and secondary resistance links. Excessive activation of adaptation reactions leads to the formation of various pathologies of inflammatory nature. Changes in the immune responses occur at all ontogenesis stages. In the present study, we conduct the experiment of induced peritonitis in animals of different ages in order to investigate more accurately adaptive responses of the immune system during inflammation.
Objectives. The aim of our research was to study changes in the indicators of adaptive humoral immunity, levels of immunoglobulin A and circulating immune complexes, phagocytic activity of neutrophils and the disruption of enzymes activity, which provide the phagocyte function in the NST test on the model of induced peritonitis in animals of different ages.
Materials and methods. The studies were performed on 200 white male rats. They were divided into a control group and the experimental rats, 3- and 22-month-old ones. Acute inflammation and dysbiosis in the small intestine were caused by intraperitoneal injection of lipopolysaccharide obtained from Escherichia coli strain. The material for the study was serum and blood elements of experimental animals.
Results. The levels of immunoglobulin A in the blood serum of 3 and 22-month-old rats with the inflammation model were reduced in comparison with this index in control group animals. The content of the CIC in the rats blood serum of both age groups was significantly higher in comparison with the control group. All the studied indices of neutrophils phagocytic activity in the 22-month-old animals with the inflammation model were lower than in the control rats of this age. In the 3-month-old rats with the inflammation model, the index of phagocytosis completeness was significantly lower in comparison with the control group. The reduction in the reserve capacity of phagocytic cells was higher in the 22-month-old animals. An increase in the neutrophils metabolic activity and a decrease in their metabolic reserve in 3 and 22-month-old rats with the inflammation model were revealed in comparison with the parameters of the control groups.
Conclusions. The results of the study indicate presence of violation of the primary cellular and secondary humoral immunity during the aging of the body and decrease in the adaptive responses of the immune system during inflammation due to an increase in antigenic effects.
Abbas, A. K., Lichtman, A. H. H., Pillai, S. (2015). Basic Immunology E-Book: Functions and Disorders of the Immune System. Elsevier Health Sciences.
Pavlov, V. A., Terrando, N. (2018). Neuro-immune interactions in inflammation and autoimmunity. Frontiers in immunology. 9. P. 772.
Pavlov, V. A., Tracey, K. J. (2017). Neural regulation of immunity: molecular mechanisms and clinical translation. Nature neuroscience. 20 (2). P. 156.
Tracey, K. J. (2018) Neurons are the inflammatory problem. Cell. 173 (5). P. 1066–1068.
Huang, Y., Chen, Z. (2016). Inflammatory bowel disease related innate immunity and adaptive immunity. American journal of translational research. 8 (6). P. 2490.
Hotamisligil, G. S. (2017.) Inflammation, metaflammation and immunometabolic disorders. Nature. 542 (7640). P. 177.
Chen, L. et al. (2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 9 (6). P. 7204.
Lamont, R. J., Hajishengallis, G. (2015). Polymicrobial synergy and dysbiosis in inflammatory disease. Trends in molecular medicine. 21 (3). P. 172–183.
Winter, S. E. (2016). Mechanisms of Dysbiosis in the Inflamed Gut. Host–Pathogen Interaction: Microbial Metabolism, Pathogenicity and Antiinfectives. P. 77–92.
Leonardi, I. et al. (2018). CX3CR1+ mononuclear phagocytes control immunity to intestinal fungi. Science. 359 (6372). P. 232–236.
Kenney, M. J., Ganta, C. K. (2014). Autonomic nervous system and immune system interactions. Comprehensive physiology. 4 (3). P. 1177–1200.
Scanzano, A., Cosentino, M. (2015). Adrenergic regulation of innate immunity: a review. Frontiers in pharmacology. 6. P. 171.
Uranov, V.N. (1995). Medical associations, medical ethics and general medical problems. M .: Publishing House of PIIMS, [in Russian].
Kundiev, Yu. I. (2006). The state of bioethics in Ukraine. Medichnij vsesvіt. 2(1-2), 41-46, [in Russian].
Komarov, F. I., Rapoport, S. I., & Chibisov, S. M. (2007). The role of the problem commission «Chronobiology and chronomedicine of the Russian Academy of Medical Sciences in the development of internal medicine» (on the 25th anniversary of creation). Klinicheskaja medicina, 85 (9), 14–16, [in Russian].
Karpishhenko, A. I. (2002). Medical laboratory technologies and diagnostics: a reference book in 2 volumes. SPb: Intermedika. 408 p, [in Russian].
Gamaleja, N. B., Mondrus, K. A. (1994). Comparison of two methods for the determination of immunoglobulin classes A, M, G (spectrophotometry and radial immunodiffusion). Klin. lab. Diagnostika. 1. P. 6–7, [in Russian].
Kudrjavickij, A. I. (1985). Evaluation of the killer bactericidal activity of neutrophils from the peripheral blood of healthy donors and patients in a direct visual test. Lab. delo. – №. 1. – P. 45–47, [in Russian].
Viksman, M. E., Majanskij, A. N. (1979). A method for evaluating the functional activity of human neutrophils by the reduction reaction of nitro blue tetrazolium, [in Russian].
Glanc, S. Et al. (1999). Biomedical statistics. M.: praktika. 459. P. 1, [in Russian].
Lapach, S. N., Chubenko, A. V., Babich P. N. (2000). Statistical methods in biomedical research using Excel. Kiev: Morion. 320. P. 2010, [in Russian].
Bondarenko, V. M. (2013.) The mechanisms of translocation of bacterial autoflora in the development of endogenous infection // Bjulleten' Orenburgskogo nauchnogo centra URO RAN. 3. P.1–21, [in Russian].
Pozur, V. V. et al. (2015) Reaction of lymphotic organisms in the presence of multi-sampling «Simbіter atsidofilny» in schur v with glutamate ozhirіnnyam. P. 50–57, [in Ukrainian].
Kozak, D. V. (2013). Dynamics of circulating immune complexes and immunoglobulins in response to polytrauma in experiment. Klinichna khirurhiia. 12. P. 76–78.
The Journal of V. N. Karazin Kharkiv National University, series Medicine has following copyright terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work, with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.