Potentialities of secondary emission mass spectrometry in control of immobilization of dyes in lipid layers and liposomes

Authors

  • O.A. Boryak Institute for Low Temperature Physics & Engineering, NASU Author
  • M.V. Kosevich Institute for Low Temperature Physics & Engineering, NASU Author
  • V.V.Chagovets Institute for Low Temperature Physics & Engineering, NASU Author
  • V.S. Shelkovsky Institute for Low Temperature Physics & Engineering, NASU Author
  • V.V. Orlov Institute for Low Temperature Physics & Engineering, NASU Author
  • V.A. Karachevtsev Institute for Low Temperature Physics & Engineering, NASU Author
  • G.P. Gorbenko V.N. Karazin Kharkov National University Author
  • Y.A. Domanov V.N. Karazin Kharkov National University Author
  • V.V. Tovstiak V.N. Karazin Kharkiv National University Author

Keywords:

lipids, dyes, immobilization, secondary ion mass spectrometry

Abstract

In the framework of a problem of immobilization of molecular components of biosensors, a mass spectrometric method of control of immobilization of dyes in lipid layer and liposomes is suggested. A criterion of successful immobilization of dyes in a lipid monolayer on a liquid matrix surface is substantiated, which consists in the simultaneous presence of the peaks corresponding to the lipids and the dyes in the secondary ion mass spectra of the system. A method of lipids identification by their low mass characteristic fragments, which can be applied using affordable analytical instruments with a limited mass range, is suggested.

 

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Author Biographies

  • O.A. Boryak, Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • M.V. Kosevich , Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • V.V.Chagovets, Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • V.S. Shelkovsky, Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • V.V. Orlov, Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • V.A. Karachevtsev, Institute for Low Temperature Physics & Engineering, NASU

    47 Lenin Ave., Kharkiv, 61103, Ukraine

  • G.P. Gorbenko, V.N. Karazin Kharkov National University

    4 Svobody Sq., Kharkiv,  61077, Ukraine

  • Y.A. Domanov, V.N. Karazin Kharkov National University

    пр. Ленина, 47, 61103, Харьков, Украина

  • V.V. Tovstiak, V.N. Karazin Kharkiv National University

    47 Lenin Ave., Kharkiv, 61103, Ukraine

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Published

2005-06-06

Issue

Section

Methods of biophysical investigations

How to Cite

Potentialities of secondary emission mass spectrometry in control of immobilization of dyes in lipid layers and liposomes . (2005). Biophysical Bulletin, 2(16), 97-105. https://periodicals.karazin.ua/biophysvisnyk/article/view/17940

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