Spectroscopic study of interactions between polymethine dyes and liposomes in aqueous solutions
Keywords:
polymethine dyes, liposome, binding constant, intermolecular interaction, thermodynamics parametersAbstract
Interaction between cationic polymethine dyes 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO), 1,1’-
dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI), 1,1’-dioctadecyl-3,3,3’,3’-
tetramethylindodicarbocyanine perchlorate (DiD) and zwitterionic liposomes of L-α -Phosphatidylcholine
from egg yolk (PC) has been studied by spectroscopic method. Analyzing changes in the dye absorption
spectra under the variation of PC concentration and using Scott equation, binding constants (Kc) and
thermodynamic parameters ( ΔG 0 , ΔH 0 и ΔS 0 ) of ―dye-to-liposome‖ complex formation have been
calculated. It has been shown that the complex formation is spontaneous and energetically favorable
process associated with the change in dye aggregate state from associates to monomer form. The main
contribution in complex formation process belongs to hydrophobic and Van der Waals forces, while the
contribution of electrostatic interactions is negligible.
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