Applicability of cyanine aggregates for detection of nucleic acids
Keywords:
nucleic acids, groove binding, J-aggregates, cyanine dyes, fluorescent probesAbstract
The peculiarities of aggregation of cyanine dye 3,3'-dimethyl-9-(2-thienyl)-thiacarbocyanine iodide (Cyan-βTh) in binary solution DMFA:TRIS-HCl buffer (pH 8) in the presence of nucleic acids (DNA and RNA) have been examined by means of optical spectroscopy. It was found that dye aggregates interact with DNA by binding to acid groove, the process which is characterized by complicated structure of aggregates' absorbance and fluorescence. Block model of Cyan-βTh aggregation on nucleic acids where the main block is dye aggregates has been proposed. Fluorescent decay curves of dye monomers and aggregates corroborating the proposed model have been obtained. The absorbance and fluorescence bands of Cyan-βTh aggregates in the presence of DNA differ from those observed in the presence of RNA. This fact allowed attributing the examined dye to a specific one for nucleic acid detection.
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