Free radical lipid peroxidation, pyrene and 1,8-anilinenapthalenesulfonate fluorescence in rat liver microsomes under the influence of γ-irradiation and alimentary factors
Keywords:
rat liver microsomes, γ-irradiation, alimentary factors, lipid peroxidation, ascorbic Fe2 -induced lipids peroxidation, fluorescent probe, pyrene, 1,8-ANSAbstract
There was studied the interference of single γ-irradiation (8 Gr dose) and unbalanced (with animal proteins and antioxidant series vitamins) food allowance and alimentary addition (black chokeberry) on pyrene, 1,8-ANS (monomer and excimer) fluorescence intensity and ascorbic Fe2+-induced lipids peroxidation (LP) intensity and on lipid hydroperoxides contents in rat liver microsomes. It was determined, that γ-irradiation of animals fed with unbalanced food allowance leads to more acute form of LP activation and microsomal membranes reconstruction with following negative charged group number decrease on membrane surface, than irradiation of rats fed with standard vivarium food allowance. The alimentary addition of black chokeberry graded LP activation and charged groups distribution changes on liver microsomes surface of irradiated rats fed with unbalanced food. Obtained data allows us to conclude that black chokeberry alimentary addition has an antioxidant and membrane protective action and it's capable to correct liver microsomes detoxication ability changes of irradiated organism, especially, irradiated animals fed with unbalanced food allowance.
Downloads
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
