A CONCOMITANT ANTIMICROBIAL ACTIVITY OF METHYLATED AND HALOGENATED GLUCOCORTICOSTEROIDS AGAINST MICROORGANISMS ISOLATED FROM THE SPUTUM OF CHILDREN WITH BRONCHIAL ASTHMA

Abstract

A concomitant antimicrobial activity of glucocorticosteroids (GCS) - prednisolone, methylprednisolone, dexamethasone, polcortolone, beclomethasone dipropionate, fluticasone propionate - for microorganisms isolated from the sputum of 135 children with bronchial asthma (BA) aged 11 ± 0,12 years was studied. In cultures was taken into account the number of isolated strains in the titer of bacteria (S. aureus, S. pyogenes, E.coli, Pr. Mirabilis, Ps. Aeruginosa, C. albicans) 10³ U/ml and above, as well as yeast micromycetes (C. albicans). The antimicrobial activity of GCS was studied by double serial dilutions method with determining of minimum inhibitory concentration (MIC, mkg/ml) with the addition of these drugs in middle and low doses. Results were measured by nephelometric method according to changes of the optical medium density on the apparatus FEC-M with wavelength of 590 nm. The Antimicrobial activity of GCS was also analyzed depending on availability in their structure the methyl group CH₃ and/or halogens - Cl, F. The greatest antimicrobial activity had fluticasone propionate, which contains in its structure two F atoms and CH₃, and the lowest activity - prednisolone. Low doses of GCS did not demonstrate bacteriostatic action and only average doses had an impact on growth of bacteria in the study. It is concluded that in children with BA should be implemented selectivity in the appointment of inhaled and oral GCS for long-term use in average doses.