PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS

  • A. S. Tkachenko
  • O. A. Nakonechna
  • V. I. Zhukov
  • T. V. Gorbach
  • M. O. Tkachenko
Keywords: gastroenterocolitis, carrageenan, rats, poly (ADP-ribose) polymerase, caspase-3, matrix metalloproteinases

Abstract

The aim of the research was to study the activity of poly (ADP-ribose) polymerase in small intestinal homogenate of rats with chronic carrageenan-induced gastroenterocolitis, as well as mechanisms of regulation of the enzyme in this pathology. Twenty Wistar Albino Glaxo rats were divided into two groups. Animals of group 1 (n = 10) consumed 1 % carrageenan solution for 28 days, which resulted in the development of gastroenterocolitis confirmed morphologically. The control group consisted of intact animals (n = 10). The activity of poly (ADP-ribose) polymerase (PARP) in the homogenate of small intestine, as well as caspase-3, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) serum levels were determined. Obtained data were statistically processed using the Mann-Whitney U test and calculating median and interquartile range (Me, 25th–75th percentile) with the help of the GraphPad Prism 5 application. The development of carrageenan-induced gastroenterocolitis was accompanied by an increase in caspase-3,
MMP-2, MMP-9 concentrations in blood serum and a decrease in the activity of PARP in small intestinal homogenates. The reduced activity of PARP in chronic carrageenan-induced gastroenterocolitis may be due to the proteolysis of this enzyme under the action of caspase-3, MMP-2, and MMP-9.

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References

1. Suicidal cross-linking of PARP-1 to AP site intermediates in cells undergoing base excision repair /
[R Prasad, JK Horton, PD Chastain, NR Gassman, BD Freudenthal, EW Hou, SH Wilson] // Nucleic Acids Res. – 2014. – Vol. 42 (10). – P. 6337–6351. DOI: 10.1093/nar/gku288.
2. The PARP family: insights into functional aspects of poly (ADP-ribose) polymerase-1 in cell growth and survival / [T Jubin, A Kadam, M Jariwala, S Bhatt, S Sutariya, AR Gani, S Gautam, R Begum] // Cell Proliferation. – 2016. – Vol. 49 (4). – P. 421–437. DOI: 10.1111/cpr.12268.
3. In vivo activated caspase-3 cleaves PARP-1 in rat liver after administration of the hepatocarcinogen N-nitrosomorpholine (NNM) generating the 85 kDa fragment / [J Wesierska-Gadek, M Gueorguieva,
J Wojciechowski, S Tudzarova-Trajkovska] // J. Cell. Biochem. – 2004. – Vol. 93, № 4. – P. 774–87.
4. PARP-1 cleavage fragments: signatures of cell-death proteases in neurodegeneration / [GV Chaitanya,
AJ Steven, PP Babu] // Cell Commun. Signal. – 2010. – Vol.8:31.
5. Gubina-Vakyulyk G. I. Damage and regeneration of small intestinal enterocytes under the influence of carrageenan induces chronic enteritis / G. I. Gubina-Vakyulyk, T. V. Gorbach, A. S. Tkachenko [et al.] // Comparative Clinical Pathology – 2015. – Vol. 24, (6). – P. 1473-1477. DOI: 10.1007/s00580-015-
2102-3.
6. Gubina-Vakulik G. I., Morfologicheskoe sostojanie tonkogo kishechnika pri dlitel'nom upotreblenii pishhevoj dobavki karraginan / G. I. Gubina-Vakulik, A. S. Tkachenko, M. A. Orlova // Vіsnik problem bіologії і medicini. – 2014. – Vol. 3 (109), Issue 2. – P. 252–256.
7. Sumbayev V. V. / V. V. Sumbayev, I. M. Yasinska, A. Y. Kosanov // Biochem. Soc. Trans. - 2000. – Vol. 28, № 5. – P.335.
8. Matrix Metalloproteinase-2 (MMP-2) gene deletion enhances MMP-9 activity, impairs PARP-1 degradation, and exacerbates hepatic ischemia and reperfusion injury in mice / H Kato, S Duarte, D Liu, RW Busuttil, AJ Coito // PloS One. – 2015. – Vol. 10 (9). DOI: 10.1371/journal.pone.0137642.
9. Tkachenko A. S. Pokazateli funkcional'nogo sostojanija jendotelija sosudov tonkogo kishechnika pri hronicheskom jeksperimental'nom gastrojenterokolite / A. S. Tkachenko // Vіsnik problem bіologії і medicini. – 2014. – Vol. 3 (112), Issue 3. – P. 204–207.
10. Tkachenko A. S. Sistema generacii oksida azota pri jeksperimental'nom gastrojenterokolite / A. S. Tkachenko, V. G. Gopkalov, M. A. Orlova // Bukovins'kij medichnij vіsnik. – 2014. – Vol. 18, № 2 (70). – P. 109–112.
11. Review of harmful gastrointestinal effects of carrageenan in animal experiments / [J. K. Tobacman] // Environmental Health Perspectives. – 2001. – Vol. 109, № 10. – P. 983–994.
Published
2017-12-28
How to Cite
Tkachenko, A. S., Nakonechna, O. A., Zhukov, V. I., Gorbach, T. V., & Tkachenko, M. O. (2017). PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS. The Journal of V. N. Karazin Kharkiv National University, Series "Medicine", (34), 41-46. Retrieved from https://periodicals.karazin.ua/medicine/article/view/10038
Section
Clinical research