The relative retention time variability evaluation for flavonoids retention on C18 chromatography columns
DOI:
https://doi.org/10.26565/2220-637X-2014-23-09Keywords:
relative retention time, variability, HPLC, octadecyl silica sorbentAbstract
In this study the variability of the relative retention times (RRT) for the components of the flavonoids model mixture have been investigated by using 7 different chromatographic columns with octadecilsilica sorbent (C18). It was founded, that the RRT variability depends on the choice of the standard peak, and they cannot be used for substances identification on the chromatogram. The using of several substances in one chromatogram as standard for RRT calculation does not lead to considerable decreasing in RRT variability.
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