Ascending elution in micellar thin layer chromatography on normal-phase sorbents: mobile phase velocity

doi:10.26565/2220-637X-2012-21-27

A. Yu. Renkevich V.N. Karazin Kharkiv National University
Alexander P. Boychenko V.N. Karazin Kharkiv National University
Lidiya P. Loginova V.N. Karazin Kharkiv National University
Artem Yu. Kulikov V.N. Karazin Kharkiv National University

DOI: https://doi.org/10.26565/2220-637X-2012-21-27

Keywords: micellar thin layer chromatography, viscosity, surface tension, Giddings model

Abstract

In this paper, physico-chemical characteristics (surface tension and viscosity) of micellar mobile phases on the basis of the cationic surfactant cetylpyridinium chloride with a concentration 0.01, 0.02 and 0.03 M with additives of ethanol, 1-propanol, 1-butanol or 1-pentanol were obtained. The effect of the properties of the mobile phase on the elution velocity in ascending mode of thin layer chromatography was investigated. It is shown that the viscosity of micellar mobile phases increases monotonically and the density decreases with increasing volume fraction of modifier in the micellar eluent. The surface tension of the micellar eluent is reduced after the addition of alcohol. This leads to the increasing of elution time in micellar TLC (MTLC). The values of the retardation factors of the test substances (azorubin, brilliant blue) practically do not depend on the fraction of modifier in the mobile phase for all alcohols except ethanol. Giddings model satisfactorily describes the motion of the mobile phase in MTLC for eluents with various concentrations of surface-active agent (surfactant) and the proportion of the modifier.

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References

Braithwaite, A. and F.J. Smith, Chromatographic Methods. 1999: Kluwer Academic Publish-ers.

Geiss, F., Fundamentals of Thin Layer Chromatography (Planar Chromatography). Chroma-tographic methods. 1987, Heidelberg: Huethig Verlag.

Giddings, J.C., G.H. Stewart, and A.L. Ruoff, Zone migration in paper chromatography. Jour-nal of Chromatography, 1960. 3: p. 239-251.

Ruoff, A.L. and J.C. Giddings, Paper geometry and flow velocity in paper chromatography Journal of Chromatography, 1960. 3: p. 438-442.

Berezkin, V.G., Development of nontraditional planar-chromatographic methods. Journal of Planar Chromatography - Modern TLC, 2008. 21(5): p. 325-329.

Berezkin, V.G., Nontraditional variants of thin layer chromatography. Russian Journal of Physical Chemistry A, 2008. 82(7): p. 1197-1201.

Berezkin, V.G., et al., Use of thin-layer chromatography with closed sorption layer for the de-termination of volatile compound. Petroleum Chemistry, 2009. 49(5): p. 401-405.

Berezkin, V.G. and S.L. Bolotov, Thin-layer chromatography with mobile phases of low vola-tility. Talanta, 1987. 34(1): p. 183-189.

Rebinder, P.A., Selected works. Surface phenomena in dispersed systems. Colloid chemistry. 1978, Moscow: Nauka. 121.

Zdziennicka, A. and B. Jan´czuk, Behavior of cationic surfactants and short chain alcohols in mixed surface layers at water–air and polymer–water interfaces with regard to polymer wet-tability. I. Adsorption at water–air interface. Journal of Colloid and Interface Science, 2010. 349: p. 374-383.

Boichenko, A.P., et al., The mobile phase motion in ascending micellar thin-layer chromatog-raphy with normal-phase plates. Journal of Planar Chromatography: Modern TLC, 2011: p. accepted for publication.