Interaction of new fluorescent ICT-dyes with lipid membranes
Keywords:
ICT dyes, liposomes, phosphatidylcholine, cardiolipin, cholesterolAbstract
The present study was undertaken to evaluate the sensitivity of newly synthesized ICT dyes to the
changes in physicochemical properties of lipid bilayer. Partitioning of ICT4 into lipid phase of the model
membranes composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with anionic lipid
cardiolipin and cholesterol was followed by the decrease of fluorescence quantum yield and shortwavelength shift of emission maximum. On the contrary, ICT2 exhibited tenfold increase of the quantum yield upon interaction with liposomes, without any shift of the emission maximum. Analysis of the partition coefficients showed that inclusion of cardiolipin and choleterol into phosphatidylcholine bilayer gives rise to increase of the ICT2 incorporation into lipid phase compared to the neat phosphatidylcholine membrane.
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