Hemoglobin binding to phospholipid membranes as revealed by pyrene fluorescence study
Keywords:
hemoglobin, model membranes, protein-lipid complexes, pyrene, fluorescence spectroscopyAbstract
In this work hemoglobin (Hb) association with lipid bilayers was investigated using fluorescent probe
pyrene. Model membranes were prepared from zwitterionic lipid phosphatidylcholine (PC), anionic lipid
phosphatidylglycerol (PG) and cholesterol (Chol). Hb-lipid binding was followed by the pyrene
fluorescence quenching. Hb-induced decrease of pyrene monomer fluorescence was followed by the
increase of relative intensities of vibronic bands. Presumably, Hb penetration into the bilayer increases
the space between neighbouring lipids and promotes water penetration into the membrane core. Pyrene
excimer emission quenching was interpreted in terms of resonance energy transfer. The greatest depth of
Hb penetration into the lipid bilayer was observed in PC vesicles. In Chol-containing liposomes sterol
condensing effect prevents deep protein penetration into the membrane. PG has an ability to stabilize lipid
bilayers due to the ordered state of its lipid tails and H-bonding interactions between lipid molecules. This
also can prevent Hb access to the inner membrane regions.
Downloads
Downloads
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
