INTERACTION OF Ni^2+ IONS WITH HOMOPOLYNUCLEOTIDES CONTAINING ADENINE AND URACIL AND THEIR MONOMERS

Authors

  • V. A. Sorokin B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov Author
  • V. A. Valeev B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov Author
  • M. V. Degtiar B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov Author
  • G. O. Gladchenko B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov Author
  • Yu. P. Blagoi B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov Author

Abstract

Interactions of Ni2+ ions with AMP, UMP, single-chain poly A, poly U, double-chain poly A poly U and three-chain poly A*2 poly U have been studied by the method of differential UV spectroscopy. The coiL->helix transition observed in polynucleotides is due to the Ni2+ ion binding to oxygen atoms of phosphate groups. The nickel coordination to bases takes place only in the case of single AMPs and single-chain poly A. The formation of a chelate N7-Ni2+-(P04) induces melting of this polymer. Melted parts form compact particles with the effective radius ~100A.

KEY WORDS: UV spectroscopy, homopolynucleotides, metal ions.

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Issue

Vol. 3 No. 5 (1999): Біофізичний вісник

Section

Molecular biophysics

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How to Cite

INTERACTION OF Ni^2+ IONS WITH HOMOPOLYNUCLEOTIDES CONTAINING ADENINE AND URACIL AND THEIR MONOMERS. (2015). Biophysical Bulletin, 3(5). https://periodicals.karazin.ua/biophysvisnyk/article/view/2482

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