The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells

Authors

  • Y. G. Shkorbatov V.N.Karazin Kharkov National University, Institute of Biology Author
  • V. A. Katrych V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies Author
  • V. N. Pasyuga V.N.Karazin Kharkov National University, Institute of Biology Author
  • E. A. Antonenko V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies Author
  • D. D. Ivanchenko V.N.Karazin Kharkov National University , Department of Theoretical Radiophysic Author
  • O. Y. Klyuchivskaya Institute of Cell Biology, National Academy of Sciences of Ukraine Author
  • R. S. Stoika Institute of Cell Biology, National Academy of Sciences of Ukraine Author

Keywords:

microwave radiation, human cell damage

Abstract

The effects of low intensity microwave radiation on the viability of the epithelial cells human buccal epithelium and chromatin state after in vitro microwave irradiation were investigatted. After the exposure to low-energy microwave radiation (frequency of 36.64 GHz and 1.85 GHz) the cell damage was assessed by staining with vital dye Hoechst 33342 and ethidium bromide . Condensation of chromatin in the cells was determined by the change in the number of granules of heterochromatin after staining with orcein in interphase nuclei. The cell damage after microwave irradiation was partially recovered after a certain period of time (from 1.5 to 4 hours) in dependence on radiation intensity. The low -energy microwave radiation also induced an increase in chromatin condensation (heterochromatinization) independence on the duration of cell exposure to microwaves

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Author Biographies

  • Y. G. Shkorbatov, V.N.Karazin Kharkov National University, Institute of Biology
    61022, pl. Svobody, 4,Ukraine
  • V. A. Katrych, V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies

    Kharkov, 61022, pl. Svobody, 4, Ukraine

  • V. N. Pasyuga, V.N.Karazin Kharkov National University, Institute of Biology

     Kharkov, 61022, pl. Svobody, 4, Ukraine

  • E. A. Antonenko, V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies

    Kharkov, 61022, pl. Svobody, 4, Ukraine

  • D. D. Ivanchenko, V.N.Karazin Kharkov National University , Department of Theoretical Radiophysic

    Kharkov, 61022, pl. Svobody, 4,Ukraine

  • O. Y. Klyuchivskaya, Institute of Cell Biology, National Academy of Sciences of Ukraine

    Lviv, 79005, ul. Drahomanova 14/16

  • R. S. Stoika, Institute of Cell Biology, National Academy of Sciences of Ukraine

    Lviv, 79005, ul. Drahomanova 14/16

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Issue

Vol. 1 No. 29 (2013): Біофізичний вісник

Section

Action of physical agents on biological objects

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How to Cite

The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells. (2015). Biophysical Bulletin, 1(29). https://periodicals.karazin.ua/biophysvisnyk/article/view/2369

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