The influence of newly synthesized pegylated carrier on the changes of na+, k+-atp-ase activity of the loach embryos during early embryogenesis
Keywords:
surface-active pegylated polymer, Na, K -АТP-ase activity, loach embryos, type of inhibition,, embryogenesisAbstract
The evaluation of influence of VEP-GMA-graft-PEG carrier in concentrations of 10-6÷10-18 М on the
Na+, K+–АТP-ase activity in the early period of development (60 – 330 min) of loach embryos has been
performed. Investigation of loach embryos morphology under the influence of BAS during early
embryogenesis, revealed no significant differences in their growth compared with controls. After
approximation of the change of Na+, K+-ATPase activity by VEP-GMA-graft-PEG exposure on the stage
of 10th division of blastomeres in concentrations of 1,5 (R2 = 0,75) and 3 (R2 = 0,94) mM ATP, nonlinear
dependence of enzyme kinetics has been established, indicating a mixed type of inhibition. According to
the performed univariate analysis of variance it has been found that changes in the Na, K-ATPase activity
of embryos were caused by the presence of polymeric carrier in the medium. Two-factor analysis of
variance revealed no significant effect of changes of VEP-GMA-graft-PEG influence on the membrane
enzyme activity, however under the presence of BAS in concentrations of 10-8÷10-6 M, reliability of the
results is the following: p> 0.95 and p>0,99
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