INFLUENCE OF γ-RADIATION ON STRUCTURAL STATE OF DNA IRRADIATED AT VARIOUS HUMIDITY LEVELS
Keywords:
DNAAbstract
A study of γ-radiation influence on calf thymus NaDNA at various relative humidity levels has been carried out with using gel-electrophoresis and UV-spectroscopy techniques. It has been shown that irradiated DNA samples differ from check sample in molecular mass (fragment lengths) distribution due to partial fragmentation of DNA molecules, the fragmentation degree depending upon relative humidity of the sample during irradiation. The most destruction of DNA is observed at minimum (0%) and maximum (90%) relative humidity, and the least one - in the interval of 56-64% relative humidity. UV-spectroscopy data give evidence of that with increase of relative humidity of DNA samples irradiated with a certain dose shift of DNA melting temperature towards its increase is observed. It is concluded that main targets of y-radiation influence on DNA are diesteric bonds between AT-pairs.
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