Hydration of actinocin derivative and its non and γ-irradiated complexes with DNA on data of ENF dielectrometry
Keywords:
actinocin derivative, complex DNA with actinicin derivative, hydration, γ-irradiation, ENF differential dielectrometric method, electrophoresisAbstract
Measurements of complex dielectric permittivity of aqueous salt solutions (0,08 M NaCl) of actinocin derivative with two methylene groups in aminoalkyl side chains (ActII) were carried out by a method of EHF differential dielectrometry. Dielectric properties of aqueous salt solutions of non and γ-irradiated at dose 800 Gy complexes of DNA-ActII (P/D=5) also were investigated. The value of gydration h (a number of water molecules per one molecule of ligand or DNA nucleotide) was calculated for all investigated substances. It was found that h=22 for ActII, h>30 for the complex DNA-ActII. γ-irradiation of the complex leads to the decrease of h by 18%. The degree of DNA fragmentation was determined by a method of electrophoresis. It was found that the irradiated complex DNA-ActII is more mobile and more fragmented than the non irradiated one.
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