Mechanism of extracellular ice nucleation in cryobiological systems

  • L. G. Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
Keywords: biological system, ice nucleation, cryomicroscopy

Abstract

Mechanism of extracellular ice nucleation in biological system on base of lyophylised human blood plasm was studied by means of small drop method and cryomicroscopy. Primary nucleation in the model system in studied overcooling range was shown to be realized almost completely by a heterogeneous mechanism, contributing 98,7% into phase transition. It was established that the nucleation frequency rises on both system cooling rate increasing and system overcooling.

Downloads

Download data is not yet available.

Author Biography

L. G. Kuleshova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine

23, Pereyaslavskaya str, 61015, Kharkov, Ukraine

References

Bakli G. Rost kristallov. M.:Inostrannaia literatura; 1954. 406p. (in Russian)

Kozlova OG. Morfologo-geneticheskii analiz kristallov. M.:MGU; 1991. 224p.

Kuleshova LG, Rozanov LF. Rol struktury vnekletochnogo lda v protcesse kriokonservirovaniia kletok. Modelirovanie kriobiologicheskikh protcessov. Kharkov; 1998. p.25-34. (in Russian)

Beliaev VI. Ob opredelenii kriticheskogo razmera zarodyshevykh kristallov. Izvestiia Akademii nauk SSSR. Seriia geofizicheskaia. 1960;8:1278-81. (in Russian)

Samygin GA. Prichiny vymerzaniia rastenii. M.:Nauka; 1974.192p. (in Russian)

Rozental OM. O rabote ldozarozhdeniia v vode. ZhSKh. 1965;6(4):635-6. (in Russian)

Turnbull D, Cech RE. Microscopic observation of the solidification of small metal droples. J. Appl. Phys. 1950;21(8):804-10.

Kozlov GA, Ravdel AA. Kinetika izotermicheskoi kristallizatcii pereokhlazhdennykh kapel vody, sostavliaiushchikh dispersnuiu fazu emulsii tipa voda-maslo. Kolloidnyi zhurnal. 1971;33(6):847-53. (in Russian)

Chernov AA, Givargizov EI, Bagdasarov KhS, Kuznetcov VA. Sovremennaia kristallografiia. Obrazovanie kristallov. M.:Nauk4a; 1980. 407p. (in Russian)

Kozlov GA, Teodorovich VI. Kinetika izotermicheskoi kristallizatcii pereokhlazhdennykh kapel krovi. Biofizika. 1970; 15(4):657-64.(in Russian)

Kozlov GA, Teodorovich VI, Ravdel AA. Ob Obrazovanii tcentrov kristalizatcii pri zamorazhivanii krovi s zashchitnymi veshchestvami. Biofizika. 1967;12(3):483-8.

Melikhov VI, Prisiazhniuk VA. O nukleatcii v dispergirovannykh zhidkostiakh. ZhFKh. 1979;43(5):1108-13. (in Russian)

Zatcepina GN. Svoistva i struktura vody. M.:MGU; 1974. 167p. (in Russian)
Published
2006-06-05
Cited
How to Cite
Kuleshova, L. G. (2006). Mechanism of extracellular ice nucleation in cryobiological systems. Biophysical Bulletin, 1(17), 65-70. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12879
Section
Biophysics of complex systems