The effects of mechanical stretching in skin on the thermostability of collagen synthesized in it
Keywords:
connective tissue, mechanical stretching, collagen, hydroxylation, glycolysation, thermostbility, denaturation
Abstract
The effects of mechanical stretching on denaturation parameters, as well as the degree of proline hydroxylation, glycolysation of lysine and hydroxylysine residues of I collagen in rat's skin have been studied in vitro. It was found that collagen synthesized in the skin under the influence of mechanical stretching, produces less thermostable fibrils than that synthesized without it. This effect appeared to be a result of decreased intensity of two enzymatic reactions of collagen primary structure processing - proline hydroxylation and glycolysation of lysine and hydroxylysine residues.
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References
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Dubous M, Gilles KA, Hamilton JK. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1986;28(3):350-6.
Burzhanadze TV. Termodinamicheskoe obosnovanie struktury kollagena. Biofizika. 1992;37(2):231-7. (in Russian)
Juliano R, Haskill S. Signal transduction from the extracellular matrix. J. Cell Biol. 1993;120:577-85.
Esipova NG, Aizenkhaber F, Aizenmenger Sh, Tumanian BG. Novaia traktovka termodinamicheskoi roli aminokislot v kollagene. Razreshenie termodinamicheskogo paradoksa. Biofizika. 1992;37(1):68-72. (in Russian)
Stephen A. Greene. Veterinary anesthesia and pain management secrets. Henlay&Belfus inc; 2002. P. 266.
Grabuzenko OB, Emetc EB, Perskii EE. Vliianie deformatcii na obmen belkov i mekhanicheskie svoistva aorty i kozhi krys in vitro. Vestn. probl. biol. i med. 1997;25:19-26. (in Russian)
Skoups R. Metody ochistki belkov. M.:Mir; 1985. p. 32,203. (in Russian)
Utevskaia LA, Perskii EE. Prostoi metod opredeleniia summarnogo i svobodnogo oksiprolina. Vestn. Khark. un-ta. 1982;226:18-20. (in Russian)
Bishop JE. Increased collagen synthesis and decreased collagen degradation in right ventricular by pressure overload. Cardiovasc. Res. 1994;28:1501-85.
Zamaraeva TV. Opredelenie 14С -oksiprolina kak metod dldia izucheniia sinteza kollagena v modelnykh sistemakh. Sovr. metody v biokhimii. M.:Meditcina; 1977. p.265-70. (in Russian)
Dubous M, Gilles KA, Hamilton JK. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1986;28(3):350-6.
Burzhanadze TV. Termodinamicheskoe obosnovanie struktury kollagena. Biofizika. 1992;37(2):231-7. (in Russian)
Published
2007-06-05
Cited
How to Cite
Zhukova, T. V., Kot, Y. G., & Persky, E. E. (2007). The effects of mechanical stretching in skin on the thermostability of collagen synthesized in it. Biophysical Bulletin, 1(18), 65-68. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12623
Section
Biophysics of complex systems
Copyright (c) 2007 Biophysical visnik
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