The effect of two binding types on parameters of helix – coil transition in polynuleotides
Keywords:
DNA, actinocin derivatives, intercalation, external binding, melting parameters, calorimetry, spectrophotometryAbstract
An interaction of actinocin derivatives Act II and Act III with DNA is studied by differential scanning calorimetry and spectrophotometry. The equilibrium concentrations of intercalational and external types of complexes in Act – DNA systems at different relative DNA concentration are calculated from spectrophotometric titration data. The binding parameters for each type of actinocin derivatives – DNA complexes are obtained. The heat capacity curves of Act – DNA complexes were obtained at different relative ligand concentrations. Calorimetric study achieved that intercalation binding type leads to increase of enthalpy, entropy and melting temperature of DNA, while the external type induces the rise of the helix thermal stability only. The comparison of helix – coil transition parameters of DNA in the complexes with actinocin derivatives and proflavine is performed. The effect of ligands on DNA melting parameters correlates to binding constants obtained by spectrophotometry.
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