Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation

Authors

  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of NASU Author

Keywords:

cryopreservation, cooling rate, rapid two-step freezing, mammalian embryo

Abstract

The research was aimed at the theoretical evaluation of the application expediency of non-linear two-step freezing regimens for mammalian embryo cryopreservation. Basing on the comparison of theoretical dependency of intracellular solution overcooling on time (or temperature) under linear and two-step freezing regimens there the advantages of the latter for mammalian embryo cryopreservation were established and explained. Theoretical evaluation of optimal cooling rate by the two-factor cryodamage theory for two-cell murine embryos coincides with experimental data

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Author Biography

  • M. V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya str., Kharkov, Ukraine 61015

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Published

2008-09-29

Issue

Vol. 1 No. 20 (2008): Біофізичний вісник

Section

Biophysics of complex systems

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How to Cite

Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation. (2008). Biophysical Bulletin, 1(20), 73-78. https://periodicals.karazin.ua/biophysvisnyk/article/view/1583