The DNA chains interaction energy in E. coli promoters in connection with their functional activity
Keywords:
promoters, promoter strength, nucleotide sequence, energy of interaction of DNA chains, physic properties of promoterAbstract
The calculations of interaction energy of complementary nucleotides in promoter were performed. The methods of graphic representation of energetic promoter properties were elaborated. The data obtained indicate that the energy distribution through the promoter length has 3 minimums centered at the positions -35, -8 and +7 nucleotide number from the starting site of transcription. The most important sequence (Pribnow box) differs from the rest of promoter in its low energy of interaction between DNA chains. We defined 4 clusters in Pribnow box and in -35 sequences. The negative correlation between the mean DNA chains interaction energy in the clusters in Pribnow box and promoter strength was determined. Quite on the contrary in -35 box the mean DNA chains interaction energy directly correlate with promoter strength.
Downloads
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
