Spectroscopic and calorimetris investigation of flavin-mononucleotide - DNA system
Keywords:
flavin - mononucleotide, DNA, calorimetry, spectrophotometry, binding parametersAbstract
The interaction of flavin – mononucleotide (FMN) with salmon testes DNA is investigated by differential
scanning calorimetry, spectrophotometry, and fluorescent spectroscopy. It is shown that the addition of FMN to
DNA doesn't change DNA melting parameters significantly. The binding constant K = 50 М-1 for the
interaction of FMN with DNA is calculated from spectrophotometric data. Such value of the binding constant
is incomparably smaller than binding constants for the biologically active ligand to DNA and the constants for
ligand - FMN heteroassotiation.
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Molecular biophysics
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How to Cite
Spectroscopic and calorimetris investigation of flavin-mononucleotide - DNA system. (2010). Biophysical Bulletin, 1(24). https://periodicals.karazin.ua/biophysvisnyk/article/view/3865
