Investigation of the influence of gamma-irradiation and freezing on the thermodenaturation of placental human serum albumin by the method of dsc
Keywords:
placental albumin, gamma-irradiation, differential scanning calorimetry, denaturationAbstract
In the paper the influence of various doses of gamma-irradiation (60-10000 Gy) on the process of the
denaturation of placental human serum albumin was investigated by the method of differential scanning
calorimetry (DSC). The combined affect of radiation and freezing down to the temperature of liquid
nitrogen on serum albumin in solution was carried out. The samples was irradiated at room temperature
on gamma-apparatus «Issledovatel» by Co60 gamma-quanta on power of the exhibition dose of 120 R/min. In the result of the study it was found that gamma-irradiation of placental albumin led to the loosening of macromolecules obviously evinced already at the dose of 1729 Gy and subsequent destruction of their structure. The lowering of cooperative processes at melting begins at low doses of radiation (60 Gy). It was showed that the cooling of albumin solutions down to the temperature of liquid nitrogen both before and after irradiation introduces the additional contribution to destructive processes taking place with albumin macromolecules.
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