Measurement of the permeability of plasma membrane of murine bone marrow cells to water and cryoprotectants

Authors

  • V.S. Kholodnyy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine Author

Keywords:

murine bone marrow cells, osmotic behaviour, Kedem Katchalsky formalism, permeability parameters, dimethyl sulfoxide, propylene glycol, glycerol

Abstract

The authors studied the osmotic behavior of murine bone marrow cells in the hypertonic solutions of permeating cryoprotectants dimethyl sulfoxide (Me2SO), propylene glycol, and glycerol. Using the Kedem Katchalsky formalism the permeability parameters of the plasma membrane for water and mentioned cryoprotectants were determined. The results demonstrate a hydraulic conductivity, Lp in the presence of cryoprotectant of 1.00 ± 0.09, 1.07 ± 0.12, and 1.19 ± 0.15 (x 10 -13 m3/s/N) and cryoprotectant permeability coefficient. K1, of 6.92 ± 0.08. 11.83±0.11 and 0.94 ±0.13 (x 10 -8 m/s) for Me2SO, propylene glycol and glycerol, respectively.

 

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Author Biography

  • V.S. Kholodnyy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine

    23, Pereyaslavskaya str., Kharkov, 61015, Ukraine

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Published

2002-06-06

Issue

Vol. 1 No. 10 (2002): Біофізичний вісник

Section

Cell biophysics

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How to Cite

Measurement of the permeability of plasma membrane of murine bone marrow cells to water and cryoprotectants. (2002). Biophysical Bulletin, 1(10), 73-77. https://periodicals.karazin.ua/biophysvisnyk/article/view/18418