Mobility of nucleobases during hydration of double-stranded polynucleotides in thin films

Authors

  • M. A. Semenov Institute of Radiophysics and Electronics NAS of Ukraine Author
  • O. P. Boryskina Institute of Radiophysics and Electronics NAS of Ukraine Author
  • V. Ya. Maleev Institute of Radiophysics and Electronics NAS of Ukraine Author

Keywords:

poly(rA)-poly(rU), poly(rG)-poly(rC), poly(dA)-poly(dT), hydration, resonance interactions, helix angle, mobility, IR spectroscopy

Abstract

The known crystal structures and experimental data of IR spectroscopy of double-stranded polynucleotides poly(rA)-poly(rU), poly(rG)-poly(rC) and poly(dA)-poly(dT) are used to calculate the resonance interactions of the stretching carbonyl C=O and intra-ring C=N vibrations of the nucleobases on the basis of the molecular exciton theory. On the basis of the calculation results and the experimental data of the frequency shifts of the Amide absorption bands of the studied nucleotides during their transition from the dehydrated to the fully hydrated state, the mobility of the nucleobases during the hydration-induced conformational transitions has been estimated. It has been shown that the double-stranded nucleobases in the dry state exist in the form, which does not differ much from the double-helical one.

Downloads

Download data is not yet available.

Author Biographies

  • M. A. Semenov, Institute of Radiophysics and Electronics NAS of Ukraine

    12 Acad. Proskura str., Kharkiv, 61085, Ukraine

  • O. P. Boryskina, Institute of Radiophysics and Electronics NAS of Ukraine

    12 Acad. Proskura str., Kharkiv, 61085, Ukraine

  • V. Ya. Maleev, Institute of Radiophysics and Electronics NAS of Ukraine

    12 Acad. Proskura str., Kharkiv, 61085, Ukraine

Downloads

Published

2007-06-05

Issue

Vol. 2 No. 19 (2007): Біофізичний вісник

Section

Molecular biophysics

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

Mobility of nucleobases during hydration of double-stranded polynucleotides in thin films. (2007). Biophysical Bulletin, 2(19), 20-28. https://periodicals.karazin.ua/biophysvisnyk/article/view/17102

Most read articles by the same author(s)

<< < 1 2 3