Study of the influence of ozone on biological systems by methods of optical spectroscopy

Authors

  • I. A. Musina Institute for Problems of Cryobiology and Cryomedicine of NASU Author
  • I. A. Belykh Institute for Problems of Cryobiology and Cryomedicine of NASU Author
  • E. V. Onishchenko Institute for Problems of Cryobiology and Cryomedicine of NASU Author
  • V. D. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of NASU Author
  • T. S. Diubko Institute for Problems of Cryobiology and Cryomedicine of NASU Author

Keywords:

ozone, spectroscopy, fluorescence, yeast, microsomes, biopolymers

Abstract

The spectral characteristics of Saccharomyces cerevisiae yeast cells, model membrane systems (microsomes), and some biopolymers (bovine serum albumin (SAB) and cholinesterase (CE)) before and after the effect of ozone have been studied. The methods of spectrofluorimetry and spectrophotometry have been used. It was shown, that the influence of ozone on studied objects has a precise dose-dependent character. The threshold value of ozone doze at which the destructive action of ozone on yeast cells starts to appear has been established. A decrease of microviscosity of annular lipids under the action of ozone was found. It was shown, that own fluorescence of CE and SAB increases at low doses of ozone. The intensity of own fluorescence of CE and SAB decreases down to the complete disappearance at high doses of ozone. It specifies the severe destruction of these molecules under the action of ozone.

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Author Biographies

  • I. A. Musina, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya St., Kharkov, 61015, Ukraine

  • I. A. Belykh, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya St., Kharkov, 61015, Ukraine

  • E. V. Onishchenko, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya St., Kharkov, 61015, Ukraine

  • V. D. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya St., Kharkov, 61015, Ukraine

  • T. S. Diubko, Institute for Problems of Cryobiology and Cryomedicine of NASU

    23 Pereyaslavskaya St., Kharkov, 61015, Ukraine

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Published

2005-06-06

Issue

Vol. 2 No. 16 (2005): Біофізичний вісник

Section

Cell biophysics

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How to Cite

Study of the influence of ozone on biological systems by methods of optical spectroscopy. (2005). Biophysical Bulletin, 2(16), 33-36. https://periodicals.karazin.ua/biophysvisnyk/article/view/13329

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