Mg2+ ion effect on conformational equilibrium of poly I-poly A-poly I triple helix in solution

Authors

  • V. A. Sorokin B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine Author
  • V. A. Valeev B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine Author
  • E. A. Andrus B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine Author
  • G. O. Gladchenko B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine Author
  • Yu. P. Blagoi B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine Author

Keywords:

polynucleotides, thermostability, metal ions

Abstract

Differential UV spectroscopy was used to study the temperature dependence of the conformational equilibrium in polyI-polyA-polyI (A2I) aqueous solutions containing Na+ (0,1M) and Mg2+ (10-5±0,005M) ions. Heating-induced disruption of the A2I triple helix is the A2I—>A+I+I transition (the 3—>1 transition) in the whole range of concentrations of these ions. The rise of this transition temperature with the increase of Mg2+ ion content is described satisfactorily with "ligands" theory. This fact permitted to evaluate the constant of Mg2+ ion binding to A2I (K=1250 M-1 for the null binding degree). An analytical expression has been obtained, stating a relation between values of constants of the magnesium binding to triple (K3) - and single-stranded (K1) polynucleotides and values of the linear density of charges on these polynucleotides. These values differ slightly that is conditioned with similarity of A2I and A2U structures.

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Author Biographies

  • V. A. Sorokin, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

    47 Lenin Ave., Kharkov 61103, Ukraine

  • V. A. Valeev, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

     47 Lenin Ave., Kharkov 61103, Ukraine

  • E. A. Andrus, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

     47 Lenin Ave., Kharkov 61103, Ukraine

  • G. O. Gladchenko, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

    47 Lenin Ave., Kharkov 61103, Ukraine

  • Yu. P. Blagoi, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

    47 Lenin Ave., Kharkov 61103, Ukraine

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Published

2018-06-25

Issue

Vol. 2 No. 13 (2003): Біофізичний вісник

Section

Molecular biophysics

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How to Cite

Mg2+ ion effect on conformational equilibrium of poly I-poly A-poly I triple helix in solution. (2018). Biophysical Bulletin, 2(13), 30-37. https://periodicals.karazin.ua/biophysvisnyk/article/view/12369

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