Expression of β-III-tubulin in the neonatal adrenal cell culture: comparison of monolayer and 3D-culture
DOI:
https://doi.org/10.26565/2075-5457-2017-28-9Keywords:
adrenal cell culture, monolayer culture, 3D-culture, cytospheres, spheroids, β-III-tubulin, neuroblastsAbstract
Some morphofunctional features of newborn piglets adrenal cell cultures obtained on the surfaces with different degrees of adhesiveness have been studied. It has been shown that during cultivation on adhesive surfaces a monolayer of fibroblast-like cells is formed (monolayer culture). When cultivated on a low-adhesive surface, the cells are assembled into three-dimensional spheroids that are in a floating state (3D culture). On adhesive surfaces, attached spheroids are eventually formed on the monolayer of fibroblast-like cells. When attached or floating spheroids are transferred to an adhesive surface migration of two morphological types of cells is observed – fibroblast-like and neuroblast-like cells. Immunocytochemical staining of neuroblasts, which migrate from both types of spheroids, showed the expression of neuronal marker β-III-tubulin. The number of fibroblast-like cells migrating from floating spheroids that were transferred to adhesive conditions is inversely proportional to the duration of the preliminary 3D culture. This approach allows obtaining a "pure" neuroblasts culture, which is uncontaminated by fibroblasts.
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