Formation of metastabil liquid phases in the isotonic solution of sodium cloraide during cooling
Keywords:
phase changes (transitions), liquid phase separation, crystallization, dispersed system, cryoprotectorsAbstract
In this paper the cooling process cryomicroscopy of 0.15 M of isotonic sodium chloride solution was conducted. It was shown that there is liquid – liquid phase change before the crystallization process. As a result, the coarse system (highly concentrated emulsion) was formed. The dispersed phase and the disperse medium in a binary system with the same qualitative chemical composition differ in concentration. Therefore, the greater is the volume ratio of the coexisting phases, the greater is the difference in their quantitative compositions. The dispersed phase, that composes the main volume in the system under investigation, should have lower NaCl concentration than the disperse medium and the initial solution. In this case it will be hypotonic (and disperse medium – hypertonic) in relation to cytoplasm of human internal environment. This physical-chemical factor, which hasn’t been considered previously, might be responsible for osmotic damage in living cells during cryopreservation of cell suspensions.
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