Actinocin derivative interaction with DNA molecules at different concentrations of monovalent alkaline metals ions Na+ and K+

Authors

  • Е. А. Minakova Institute of Radiophysics and Electronics of NAS of Ukraine Author
  • Е. B. Kruglova Institute of Radiophysics and Electronics of NAS of Ukraine Author

Keywords:

spectrophotometry, native thymus DNA, actinocin derivative, ionic forces, complexation parameters

Abstract

Spectrophotometric titration method is used to study the actinomycin derivative ActII*, an analog of the
actinomycin D, interaction with calf thymus DNA. Models of several types of complexes forming between
ligand and polynucleotide matrix are considered. DALSMOD optimization program of spectrophotometric
concentration dependencies are used to choose the optimal complexation model. The results of the calculation are evidence of the three different spectral types of complexes in the system Act II* -DNA. Na+and K+ions are considered as competitors of the ligand by one of the binding sites. Logarithms of binding constants of each type of complexes depend linearly on the logarithm of NaCl and KCl concentrations in the considered interval. The slopes of these dependencies are different for Na+and K+ions.

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Author Biographies

  • Е. А. Minakova, Institute of Radiophysics and Electronics of NAS of Ukraine

    Ak. Proskuri str., 12, Kharkov , 61085

  • Е. B. Kruglova, Institute of Radiophysics and Electronics of NAS of Ukraine

    Ak. Proskuri str., 12, Kharkov , 61085

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Published

2009-06-05

Issue

Vol. 2 No. 23 (2009): Біофізичний вісник

Section

Molecular biophysics

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How to Cite

Actinocin derivative interaction with DNA molecules at different concentrations of monovalent alkaline metals ions Na+ and K+. (2009). Biophysical Bulletin, 2(23), 30-39. https://periodicals.karazin.ua/biophysvisnyk/article/view/4269