Interaction of the polyhexamethyleneguanidine salts with lipid componentes of membranes
Keywords:
polyhexamethyleneguanidine, disinfectant, membrane destruction, lipids, massspectrometryAbstract
Possibilities of formation in model systems of persistent complexes between the molecules of salts
polyhexamethyleneguanidine (PНMG) and individual lipid components of the cytoplasm membrane
(CPM) were studied. We used cholesterol and phosphatidylcholines which contain fatty acids from С14:0
to С18:0. The polialkilenguanidine PHMG belongs to group of cationic disinfectants. Salts of PHMG
chloride, succinate, maleate and valerate in the experiments were used. PHMG salts in the mass spectrum
MALDI-TOF MS and TOF-PDMS are presented as oligomers (n = 2 - 10) with molecular weights from
300 to 1430 Da. Mass spectrometric studies mixtures of polycations with lipids are showed that in the
liquid state, regardless of the exposure time, in the range to m/z 3000 persistent to formation of
intermolecular between oligomers of PHMG and lipids are absent. We have tested hypothesis, according
to which a possible mechanism of the action PHMG destructive membrane in damage of the cell, can be
caused with individual phospholipids molecules destruction. Results showed the absence of lipase activity
of the preparation. These data allow us to better understand the biophysical aspects of the bactericidal
properties of PHMG and to clarify the mechanisms of its destructive effect to membrane. Phospholipids
bilayer of the CPM is the main target for the drug
Downloads
Downloads
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
