Influence of incubation medium composition on the level of follicles viability at hypothermic storage

Authors

  • К. О. Мединець
  • В. В. Кірошка
  • Ю. О. Тіщенко
  • Т. П. Бондаренко

Keywords:

hypothermic storage; viability; ovarian tissue; electrolyte; nonelectrolyte

Abstract

Viability of ovarian tissue follicles after hypothermic storage in media of different composition has been studied. Maximal resistance of cells has been revealed when we used mannitol containing solution as the incubation medium. Addition of 10% fetal bovine serum (FBS) in all solutions increases follicles viability for 10–15 %.

References

Лабораторные методы исследования в клинике: Справочник / Под ред. В.В.Меньшиковой. – М.: Медицина, 1987. – 368с. /Laboratornyye metody issledovaniya v klinike: Spravochnik / Pod red. V.V.Men'shikovoy. – M.: Meditsina, 1987. – 368s./

Durrant B.S., Pratt N.C., Russ K.D., Bolamba D. Isolation and characterization of canine advanced preantral and early antral follicles // Theriogenology – 1998. – Vol.49. – Р. 917–932.

Hyon S.H., Kim D.H. Long-term preservation of rat pancreatic islets under physiological conditions // J. Biotechnol. – 2001. – Vol.85, №3. – Р. 241–246.

JongYeob Choi, Jin-young Lee, EunYoung Lee et al. Cryopreservation of the mouse ovary inhibits the onset of primordial follicle development // Cryobiology. – 2007. – Vol.54. – Р. 55–62.

Neto V., Buff S., Lornage J. et al. Effects of different freezing parameters on the morphology and viability of preantral follicles after cryopreservation of doe rabbit ovarian tissue // Fertility and Sterility. – 2008. – Vol.89. – Р. 1348–1356.

Puehler Th., Gleich O., Schopka S. et al. Impact of normothermic perfusion and protein supplementation on human endothelial cell function during organ preservation // Ann. Thorac. Surg. – 2010. – Vol.89. – Р. 512–521.

Rajah R., Glaser E.M., Hirshfield A.N. The changing architecture of the neonatal rat ovary during histogenesis // Development dynamics. – 1992. – №195. – P. 177–192.

Rauen U., Groot H. New insights into the cellular and molecular mechanisms of cold storage injury // J. Investig. Med. – 2004. – Vol.52, №5. – P. 299–309.

Santos S.D., Biondi F.C., Cordeiro M.S. et al. Isolation, follicular density, and culture of preantral follicles of buffalo fetuses of different ages // Anim. Reprod. Sci. – 2006. – Vol.95. – Р. 1–15.

Solano R., Armas R., Pupo C.A., Castro F.O. Short term preservation of intrafollicular oocytes at 4°C // Theriogenology. – 1994. – Vol.41. – Р. 299–315.

Songsasen N., Fickes A., Pukazhenthi B.S., Wildt D.E. Follicular morphology, oocyte diameter and localisation of fibroblast growth factors in the domestic dog ovary // Reprod. Dom. Anim. – 2009. – Vol.44, №2. – Р. 65–70.

Tsuribe P.M., Gobbo C.A., Landim-Alvarenga F.C. Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue // Fertility and Sterility. – 2009. – Vol.91, №5. – Р. 1976–1983.

Wulhfard S., Tissot St., Bouchet S. et al. Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells // Biotechnol. Prog. – 2008. – Vol.24. – Р. 458–465.

Yang Y., Honaramooz A. Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells // Reproduction, Fertility and Development. – 2010. – Vol.22. – Р. 523–532.

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Published

2013-12-27

Issue

Section

CRYOBIOLOGY

How to Cite

Influence of incubation medium composition on the level of follicles viability at hypothermic storage. (2013). The Journal of V.N.Karazin Kharkiv National University. Series «Biology», 17(1056), 141-147. https://periodicals.karazin.ua/biology/article/view/13792

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