Validation of SCAR marker linked with the gene of pollen fertility restorer in sunflower

В. М. Попов

Keywords: sunflower; SCAR marker; gene of pollen fertility restorer

Abstract

The breeding lines, lines of mutant origins and samples of interspecific hybrids of sunflower have been analyzed for the presence of locus HRG01, which is linked with the gene Rf₁. The locus HRG01 has always identified when this gene is present in the plant material. In the result of amplification the amplicon with the size of 426 bp has been synthesized. In the absence of the Rf₁ gene the specific amplicon has not been synthesized in the samples of sunflower. Therefore, amplification product was not synthesized in female sunflower lines, while its synthesis was in male lines (pollen fertility restorer). In interspecific hybrids percentage of samples with HRG01 locus was 0.62.

Downloads

Download data is not yet available.

References

Ведмедева Е.В., Толмачев В.В. Генетика морфологических признаков: состояние и перспективы // Генетические ресурсы растений. – 2006. – №3. – С. 7–22. /Vedmedeva Ye.V., Tolmachev V.V. Genetika morfologicheskikh priznakov: sostoyaniye i perspektivy // Geneticheskiye resursy rasteniy. – 2006. – №3. – S. 7–22./

Гаврилова В.А., Анисимова И.Н. Генетика культурных растений. Подсолнечник. – СПб., 2003. – 204с. /Gavrilova V.A., Anisimova I.N. Genetika kul'turnyyh rasteniy. Podsolnechnik. – SPb., 2003. – 204s./

Кириченко В.В., Сивенко В.І., Макляк К.М. та ін. Вирощування насіння гібридів соняшнику (методичні рекомендації). – Харків, 2014. – 28с. /Kirichenko V.V., Sivenko V.І., Maklyak K.M. ta іn. Vyroshchuvannya nasіnnya gіbrydіv sonyashnyku (metodychnі rekomendatsіyi). – Kharkіv, 2014. – 28s./

Попов В.Н., Кириченко В.В. Мужская стерильность подсолнечника. – Харьков: ИР, 2010. – 156с. /Popov V.N., Kirichenko V.V. Muzhskaya steril'nost' podsolnechnika. – Khar'kov: IR, 2010. – 156s./

Шарыпина Я.Ю., Попов В.Н., Долгова Т.А., Кириченко В.В. Изучение наследования морфологических признаков подсолнечника. I. Генетический контроль окраски ложноязычковых цветков, ветвистости и фертильности пыльцы // Цитология и генетика. – 2008. – Т.42, №5. – С. 47–53. /Sharypina Ya.Yu., Popov V.N., Dolgova T.A., Kirichenko V.V. Izucheniye nasledovaniya morfologicheskikh priznakov podsolnechnika. I. Geneticheskiy kontrol' okraski lozhnoyazychkovykh tsvetkov, vetvistosti i fertil'nosti pyl'tsy // Tsitologiya i genetika. – 2008. – T.42, №5. – S. 47–53./

Gupta P., Varshney R., Sharma P., Ramesh B. Molecular markers and their application in wheat breeding // Plant Breeding. – 1999. – Vol.118. – P. 369–390.

Heesacker A., Kishore V., Gao W. et al. SSRs and INDELs mined from the sunflower EST database: abundance, polymorphism and cross-taxa utility // Theor. Appl.Genet. – 2008. – Vol.117. – P. 1021–1029.

Horn R., Fried W. CMS sources in sunflower: different origin but same mechanism? // Theor. Appl. Genet. – 1999. – Vol.98. – P. 195–201.

Horn R., Kusterer B., Lazarescu E. et al. Molecular mapping of the Rf1 gene restoring pollen fertility in PET1-based F1 hybrids in sunflower // Theor. Appl. Genet. – 2003. – Vol.106. – P. 599–606.

Jan C-C., Vick B., Miller J. et al. Construction of an RFLP linkage map for cultivated sunflower // Theor. Appl. Genet. – 1998. – Vol.96. – P. 15–22.

Lai Z., Livingstone K., Zou Y. et al. Identification and mapping of SNPs from ESTs in sunflower // Theor. Appl. Genet. – 2005. – Vol.111. – P. 1532–1544.

Kusterer B., Horn R, Friedt W. Molecular mapping of the fertility restoration locus Rf1 in sunflower and development of diagnostic markers for the restorer gene // Euphytica. – 2005. – Vol.143. – P. 35–42.

Moneger F., Smart C.J., Leaver C.J. Nuclear restoration of cytoplasmic male sterility in sunflower is associated with the tissue-specific regulation of a novel mitochondrial gene // EMBO Journal. – 1994. – Vol.13. – P. 8–17.

Schnabel U., Engelmann U., Horn R. Development of markers for use of the PEF1 cytoplasm in sunflower hybrid breeding // Plant Breed. – 2008. – Vol.127. – P. 582–591.

Tang S., Yu J K., Slabaugh M.B. et al. Simple sequence repeat map of the sunflower genome // Theor. Appl. Genet. – 2002. – Vol.105. – P. 1124–1136.

Yue B., Vick B., Cai X., Hu J. Genetic mapping for the Rf1 (fertility restoration) gene in sunflower (Helianthus annuus L.) by SSR and TRAP markers // Plant Breeding. – 2010. – Vol.129. – P. 24–28.