Cultivation of adhesive cell cultures on soft contact lenses
Abstract
The influence of physical and chemical properties of soft contact lenses on adhesion processes and proliferation of mesenchymal stem cells, allogenic fibroblasts and corneal limbal stem cells has been studied in vitro. Cell lines were cultured on the inner surface of the soft contact lenses of firms "Akvalan" (Ukraine) with a moisture content up to 50%, «Focus» (USA) – up to 90%, «Baush & Lomb» (USA) – up to 55%, «Ciba Vision» (Germany) – 35%. There have been obtained homogeneous cultures of plastic-adherent fibroblasts, mesenchymal stem cells and limbal corneal epithelial cells, their identification and proof of stemness have been performed during culturing. Obtaining confluent monolayer on the inner surface of soft contact lens needs a certain ratio of properties, such as oxygen permeability and water content, as well as proper growing medium. The objective has been realized by lenses «Ciba Vision» with a moisture content of 35% in a culture medium supplemented with growth factors.
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