Cultivation of adhesive cell cultures on soft contact lenses

  • А. С. Кавеліна
Keywords: soft contact lenses; mesenchymal stem cells; fibroblasts; limbal stem cells

Abstract

The influence of physical and chemical properties of soft contact lenses on adhesion processes and proliferation of mesenchymal stem cells, allogenic fibroblasts and corneal limbal stem cells has been studied in vitro. Cell lines were cultured on the inner surface of the soft contact lenses of firms "Akvalan" (Ukraine) with a moisture content up to 50%, «Focus» (USA) – up to 90%, «Baush & Lomb» (USA) – up to 55%, «Ciba Vision» (Germany) – 35%. There have been obtained homogeneous cultures of plastic-adherent fibroblasts, mesenchymal stem cells and limbal corneal epithelial cells, their identification and proof of stemness have been performed during culturing. Obtaining confluent monolayer on the inner surface of soft contact lens needs a certain ratio of properties, such as oxygen permeability and water content, as well as proper growing medium. The objective has been realized by lenses «Ciba Vision» with a moisture content of 35% in a culture medium supplemented with growth factors.

Downloads

Download data is not yet available.

References

Гринь В.К., Попандопуло А.Г., Кавеліна А.С. та ін. Спосіб отримання культивованих лімбальних клітин. Пат. України №65507. Заявл. 04.05.2011. Опубл. 12.12.2011. Бюл. №23. /Gryn' V.K., Popandopulo A.G., Kavelіna A.S. ta іn. Sposіb otrymannya kul'tyvovanykh lіmbal'nykh klіtyn. Pat. Ukrainy №65507. Zayavl. 04.05.2011. Opubl. 12.12.2011. Byul. №23./

Попандопуло А.Г., Кавеліна Г.С., Іванова О.М., Дрожжина Г.І. Спосіб отримання моношару культивованих клітин рогівки на внутрішній поверхні м′яких контактних лінз. Пат. №86892 України, МПК A61F9/00. Заявл. 06.08.13. Опубл. 10.01.2014. Бюл. №1. /Popandopulo A.G., Kavelіna G.S., Ivanova O.M., Drozhzhina G.І. Sposіb otrymannya monosharu kul'tyvovanykh klіtyn rogіvky na vnutrіshnіy poverkhnі m′yakykh kontaktnykh lіnz. Pat. №86892 Ukrainy, MPK A61F9/00. Zayavl. 06.08.13. Opubl. 10.01.2014. Byul. №1./

Степанов В.К. Комплексное лечение больных гнойными кератитами и их последствия. Автореф. дис. … д-ра мед. наук. – Москва, 2006. – 293с. /Stepanov V.K. Kompleksnoye lecheniye bol'nykh gnoynymi keratitami i ikh posledstviya. Avtoref. dis. … d-ra med. nauk. – Moskva, 2006. – 293s./

Ченцова Е.В., Петриашвили Г.Г., Арутюнова И.Р. Применение фетальных клеток роговицы человека для лечения различной патологии органа зрения и др. // Офтальмохирургия. – 1999. – №4. – C. 3–9. /Chentsova Ye.V., Petriashvili G.G., Arutyunova I.R. Primeneniye fetal'nykh kletok rogovitsy cheloveka dlya lecheniya razlichnoy patologii organa zreniya i dr. // Oftal'mokhirurgiya. – 1999. – №4. – C. 3–9./

Фрешни Р.Я. Культура животных клеток: практическое руководство. – Москва: Бином. Лаборатория знаний, 2010. – 691с. /Freshni R.Ya. Kul'tura zhyvotnykh kletok: prakticheskoye rukovodstvo. – Moskva: Binom. Laboratoriya znaniy, 2010. – 691s./

Appasani K., Appasani R. Stem cells & regenerative medicine: from molecular embryology to tissue engineering. – Springer Science+Busness Media, LLC, 2010. – 629p.

Dua H., Shanmuganathan V., Powell-Richards A. et al. Limbal epithelial crypts: a novel anatomical structure and a putative limbal stem cell niche // Br. J. Ophthalmol. – 2005. – Vol.89. – P. 529–532.

Garfias Y., Nieves-Hernandez J., Garcia-Mejia M. et al. Stem cells isolated from the human stromal limbus possess immunosuppressant properties // Mol. Vis. – 2012. – P. 2087–2095.

Numata R., Okumura N., Nakahara M. et al. Cultivation of corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells // PLoS ONE. – 2014. – Vol.9 (2). – e88169.

Holan V., Pokorna K., Prochazkova J. et al. Immunoregulatory properties of mouse limbal stem cells // The Journal of Immunology. – 2010. – P. 2124–2129.

Liu J., Sheha H., Fu Y. et al. Update on amniotic membrane transplantation // Expert. Rev. Ophthalmol. – 2010. – №5. – P. 645–661.

Polisetty N., Fatima A., Madhira S. et al. Mesenchymal cells from limbal stroma of human eye // Mol. Vis. – 2008. – P. 431–442.

Scalise A., Pierangeli M., Di Benedetto G. et al. Cultured autologous fibroblast and keratinocyte grafts: applications in plastic surgery // Annals of Burns and Fire Disasters. – 2001. – Vol.14, №2. – P. 96–99.

Umemoto T., Yamato M., Nishida K. et al. Limbal epithelial side-population cells have stem cell-like properties, including quiescent state // Stem Cells. – 2006. – №24 (1). – P. 86–94.

Published
2014-10-22
Cited
How to Cite
Кавеліна, А. С. (2014). Cultivation of adhesive cell cultures on soft contact lenses. The Journal of V.N.Karazin Kharkiv National University. Series «Biology», 22(1126), 8-15. Retrieved from https://periodicals.karazin.ua/biology/article/view/3970
Section
BIOTECHNOLOGY