LEUKOCYTES AS MARKERS OF HEMORRHAGIC STROKE INFLAMMATORY NATURЕ

Authors

  • A. N. Yabluchanskiy

Keywords:

hemorrhagic stroke, inflammation, collagenase-induced hemorrhagic stroke, experimental model, leukocytes, neutrophils, polymorphonuclears, mononuclears, micrgoglia, macrophages

Abstract

Hemorrhagic stroke in response to intracerebral hemorrhage is considered an aseptic form of inflammation. With this in mind the dynamics of leukocytes changes in blood and in the hemorrhagic stroke zone as main factors of inflammation are examined. Sprague-Dawley rats (N = 24) underwent surgical procedures and were divided into two groups — control group and a hemorrhagic stroke group — an injection of 2 µL of sterile saline and 2 µL of 0,2U of Collagenase-IV (S) respectively. White blood cells count analysis, combined histological and immunohistological examination with further densitometric and statistical analysis of blood and hemorrhagic stroke zone leukocytes were performed. A synchronized increase in blood and hemorrhagic stroke zone levels of both polymorphonuclear and mononuclear leukocytes was observed, highlighting the inflammatory nature of hemorrhagic stroke. A delay of 48 hours between peak levels of polymorphonuclear and mononuclear leuokocytosis both in blood and the hemorrhagic stroke zone was established. Therefore, understanding hemorrhagic stroke as one of the forms of aseptic inflammation suggests alternate perspectives in the development of new approaches to diagnosis and treatment including interventions into kinetics of blood and hemorrhagic stroke zone leukocytes.

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Issue

No. 23 (2012)

Section

Fundamental research

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How to Cite

LEUKOCYTES AS MARKERS OF HEMORRHAGIC STROKE INFLAMMATORY NATURЕ. (2015). The Journal of V. N. Karazin Kharkiv National University, Series "Medicine", 23, 10-21. https://periodicals.karazin.ua/medicine/article/view/6721