Use of Biotesting Methods for Assessing the Ecological Condition of Surface Waters

  • A. M. Krainiukova Scientific-research establishment «Ukrainian Research Institute of Environmental Problems», st. Bakulina, 6, 61166, Kharkiv, Ukraine https://orcid.org/0000-0002-1005-8850
  • O. M. Krainiukov V. N. Karazin Kharkiv National University, 6, Svobody Sqr., 61022, Kharkiv, Ukraine https://orcid.org/0000-0002-5264-3118
  • I. A. Kryvytska V. N. Karazin Kharkiv National University, 6, Svobody Sqr., 61022, Kharkiv, Ukraine https://orcid.org/0000-0003-4727-794X
Keywords: surface waters, water body, aquatic ecosystem, chemical pollution, biotesting method, toxic chemicals, ecological responsibility

Abstract

Purpose. Analysis of national and foreign experience in solving the problem of chemical pollution of surface waters and its economic consequences in accordance with the provisions of European legislation.

Methods. System analysis.

Results. Selection of the optimal set of biotesting methods for assessing the environmental consequences of chemical pollution of surface waters; study of the system of environmental liability, which operates in European and other foreign countries, and national regulations on the recovery of damages for violations of water legislation. The analysis of foreign and national sources on the use of biotesting techniques to assess the ecological status of surface waters and determine the toxic properties of water and chemicals. In world practice, a biotesting method is used to obtain data on the effects of hazardous toxic chemicals on aquatic ecosystems. Biotests are available and cheap (when using specially designed modifications for practical needs), do not require special training of performers and can be easily mastered in practical laboratories.

Conclusions. To assess and control the quality of surface waters and their sources of pollution in accordance with the recommendations of the Water Framework Directive 2000/60 / EC, biotesting techniques are used using a "basic set of taxa" - algae, crustaceans and fish.

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Author Biographies

A. M. Krainiukova , Scientific-research establishment «Ukrainian Research Institute of Environmental Problems», st. Bakulina, 6, 61166, Kharkiv, Ukraine

Doctor of Biological Sciences, Professor, Head of the Laboratory of Biological Research and Biotesting

O. M. Krainiukov , V. N. Karazin Kharkiv National University, 6, Svobody Sqr., 61022, Kharkiv, Ukraine

Doctor of Geographical Sciences, Professor, Professor of the Department of Environmental Safety and Environmental Education

I. A. Kryvytska , V. N. Karazin Kharkiv National University, 6, Svobody Sqr., 61022, Kharkiv, Ukraine

Candidate of Biological Sciences, Associate Professor of Environmental Safety and Environmental Education

References

Krainiukov, O. (2013.) Scientific and methodical bases of normalization of anthropogenic pollution of aquatic landscapes: monograph. Kharkiv: Ecograph. (In Ukrainian).

Krainiukov, O., Kryvytska, I., Krainiukov, A. & Timchenko V. (2020). Analysis of Methodological Ap-proaches to Assessing Economic Consequences of Anthropogenic Environmental Pollution. Proceed-ings of the 36nd International Business Information Management Association Conference, IBIMA. 792-784. Retrieved from https://ibima.org/university/v-n-karazin-kharkiv-national-university-ukraine/

Krainyukova, A.M., Krainyukov, O.M. & Kryvytska, I.A. (2020). Use of photosynthetic activity of algae to assess toxicity in order to create a portable device. Visnyk of V. N. Karazin Kharkiv National Uni-versity series «Еcоlogy», (22), 82-92. https://doi.org/10.26565/1992-4259-2020-22-08 (In Ukrainian).

Klimenko, M.O., Pilipenko, Y.V., Bedunkova, O.O. & Kononchuk, V.O. (2017). Respiratory intensity of Amatitlania nigrofasciata at different concentrations of toxicants in model experiments. Biological studies, 11(1), 147–160. https://doi.org/10.30970/sbi.1101.518 (in Ukrainian)

Yanovich, D.O. & Gritsynyak, I.I. (2016). Shvets TM Use of salmon (Salmonidae) in biomonitoring of aquatic environment quality. Fisheries science of Ukraine, (1 (35)), 5–30. (In Ukrainian).

Krainyukov, O.M., Strian, K.O. & Krainyukov, O.O. (2018). Establishment of metrological characteris-tics of biotesting methods for determining the acute lethal toxicity of water in fish Brachydanio rerio Hamilton-Buchanan. Young Scientist, (9), 279-282. Retrieved from http://nbuv.gov.ua/UJRN/molv_2018_9(2)__3 (In Ukrainian).

Kryvytska, I.A. & Krainyukov, A.A. (2019). Establishment of metrological characteristics of the method of biotesting to determine the acute lethal toxicity of water on crustaceans Ceriodaphnia affinis Lill-jeborg. Young Scientist, (2), 323-326. Retrieved from http://nbuv.gov.ua/UJRN/molv_2019_2(2)__8. (In Ukrainian).

Krainyukov, O.M. (2017). Establishment of standards for maximum permissible levels of toxicity of wastewater based on the application of constructive-geographical methodology of subject-object rela-tions. Visnyk of V. N. Karazin Kharkiv National University series «Еcоlogy», (16), 22-29. Retrieved from http://journals.uran.ua/visnukkhnu_ecology/article/view/109335 (In Ukrainian).

Krainyukov, O.M. & Timchenko, V.D. (2019). Methodological principles of constructive geography in the study of the state and protection of natural landscapes. Man and environment. Issues of neoecolo-gy. (4 (31)), 6-16. Retrieved from http://journals.uran.ua/ludina_dov/article/view/186771 (In Ukrainian).

Peltier, W. H. (1986). Impact of an industrial effluent on aquatic organisms: EPA region IV case histo-ry. Proc. Pellston Environ. Workshop, Cody, Wyo., 1982, 22 - 27 Aug.. (pp. 216 – 227). New York..

Water research strategy. (1982). Environmental Protection Agency: DC - 10460. Washington.

Lewis, P.A. Klemm, D.J., Lazorchak J.M. (Eds.). (1994). Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. [3rd Ed.]. USEPA/600/4-91/002.

Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part 136). EPA 821-B-00-004. 2000. Retrieved from https://www.epa.gov/sites/production/files/2016-02/documents/method-guidance-recommendations-wet-testing_2000.pdf

Metal Mining Effluent Regulations (SOR/2002-222). Canada. Retrieved from https://laws.justice.gc.ca

Wastewater Systems Effluent Regulations (SOR/2012-139). Canada. Retrieved from https://laws.justice.gc.ca

Pulp and Paper Effluent Regulations (SOR/92-269). Canada. Retrieved from https://laws.justice.gc.ca

Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Retrieved from http://data.europa.eu/eli/dir/2000/60/oj

Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on envi-ronmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council. Retrieved from http://data.europa.eu/eli/dir/2008/105/oj

Directive 2013/39/EU of the European Parliament and of the Council of 12 August 2013 amending Directives 2000/60/EC and 2008/105/EC as regards priority substances in the field of water policy Text with EEA relevance. Retrieved from http://data.europa.eu/eli/dir/2013/39/oj

Directive 2004/35/CE of the European Parliament and of the Council of 21 April 2004 on environmen-tal liability with regard to the prevention and remedying of environmental damage. Retrieved from http://data.europa.eu/eli/dir/2004/35/oj

Directive 2009/128/EC of the European Parliament and of the Council of 21 October 2009 establishing a framework for Community action to achieve the sustainable use of pesticide. Retrieved from http://data.europa.eu/eli/dir/2009/128/oj

Directive 2010/75/EU of the European Parliament and of the Council of 24 November 2010 on indus-trial emissions (integrated pollution prevention and control). Retrieved from http://data.europa.eu/eli/dir/2010/75/oj

Regulation (EC) No 166/2006 of the European Parliament and of the Council of 18 January 2006 con-cerning the establishment of a European Pollutant Release and Transfer Register and amending Council Directives 91/689/EEC and 96/61/EC. Retrieved from http://data.europa.eu/eli/reg/2006/166/oj

Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), estab-lishing a European Chemicals Agency, amending Directive 1999/45/EC and repealing Council Regula-tion (EEC) No 793/93 and Commission Regulation (EC) No 1488/94 as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC. Re-trieved from http://data.europa.eu/eli/reg/2006/1907/oj

Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Direc-tives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006. Retrieved from http://data.europa.eu/eli/reg/2008/1272/oj

Vassuer, P. .Férard, J.F & Babut, M. (1991). The biological aspects of the regulatory control of indus-trial effluents in France. Chemosphere, 22 (5–6), 625-633. Retrieved from https://www.sciencedirect.com/science/article/abs/pii/004565359190073M

Vullierment, B. (1980). Improvement of the mass end energy balances in the tanning industry. Ala. Acad. Sci.,75, 233 - 275.

Keddy, C. I. (1995). Ecotoxicol. Environ. Saf., (3), 221-251. https://doi.org/10.1006/eesa.1995.1027

Klein, L. (1957). Aspects of River Pollution. London, 1957. 621 p.

Kolupaev, B.I. (1984). Research on aquatic toxicology in Sweden. Hydrobiol. Magazine, 20 (1), 97-107.

Miettinen, V. (1986). The role of aquatic toxicology in water pollution control. Roporttisar Ioensuun yliopisto Matluonnoutieteellis tiedekuunan, (8), 41 - 42.

Nikunen, E. (1985). Daphnia magna as indicator of the acute toxicity of waste waters. Bull. Environ. Contam. and Toxicol., (3), 368 - 374. https://doi.org/10.1007/BF01636524

Pascal, D. (1987). The role of aquatic toxicity tests in predicting and monitoring pollution effects. Acta Biologica Limnologica Hungarica, (1), 47-58.

Water Code of Ukraine. (1995). Vidomosti Verkhovnoi Rady Ukrainy, (24),.189. Retrieved from https://zakon.rada.gov.ua/laws/show/213/95-%D0%B2%D1%80

About the statement of the Order of development of specifications of maximum admissible dumping of polluting substances in water objects and the list of polluting substances which dumping in water ob-jects is standardized. (2017). Resolution of the Cabinet of Ministers of Ukraine № 1100 of 11.09.96 (as amended in accordance with the resolution of the Cabinet of Ministers of Ukraine of 13 December 2017 № 1091). Retrieved from https://zakon.rada.gov.ua/laws/show/1100-96-%D0%BF

Instruction on the procedure for development and approval of maximum permissible discharges (MPD) of substances into water bodies with return waters .(1994). Approved by the order of the Minister of Environmental Protection of Ukraine dated 15.12.94 № 116. Registered in the Ministry of Justice of Ukraine on 22.12.94 for № 313/523. Retrieved from https://zakon.rada.gov.ua/laws/show/z0313-94#Text

Methods for determining the levels of toxicity of surface and return waters to control compliance with their quality to regulatory requirements. (2000). Approved by the order of the Ministry of Ecological Security of Ukraine dated 31.01.2000 № 27. Kyiv: Ministry of Ecological Security of Ukraine. (In Ukrainian).

Krainiukova, A.N. (1991). Biotesting in the system of assessment and control of sources of toxic pollu-tion of the aquatic environment. Extended abstract of Doctor’s thesis: 14.00.20 "Toxicology". Kupav-na, (In Russian)

Comparative evaluation of biotesting methods approved in Ukraine and combined into a Watertox battery, in order to create a set of biotests that are most optimal for local conditions. (2000). Project Report № 91/13 for the International Center for the Development of Scientific Research, Canada. Kyiv. (In Ukrainian).

Arkhipchuk, V.V. (2008). Research in the field of cytogenetics of fish and biotesting. Kiev: Relics. (In Ukrainian).

Krainiukov, O.M. (2013). Criteria for assessing the sensitivity of organisms and the effectiveness of biotesting methods to determine the toxic properties of water. Visnyk of V. N. Karazin Kharkiv National University series «Еcоlogy», (1012), 64-69. Retrieved from http://journals.uran.ua/visnukkhnu_ecology/article/view/21147 (In Ukrainian).

Krainiukov, O.M. & Krainiukova, A.M. (2009). Device for biological water testing. Patent of Ukraine for useful model. GO1N 33/18. № 45811; declared 25.11.2009. Bulletin, (12). (In Ukrainian).

Implementation of the results of toxicological monitoring in the system of ecological management of the Dnieper river basin.(1999). Project Report № 91/12 for the International Center for the Develop-ment of Scientific Research, Canada. UKRNDIEP: Kharkiv. (In Ukrainian).

Results of ecotoxicological assessment of the state of transboundary rivers of Lviv, Zakarpattia and Volyn regions and sources of their pollution. (2000). Scientific and technical report on the implemen-tation of work on the program SHS TACIS Bug and Latorytsia . Uzh. - Lviv-Uzhhorod-Kharkiv. (In Ukrainian).

Krainiukova, A. N. (2011). Comprehensive assessment of the ecological status of water bodies (on the example of the basin of the North Donets River). Visnyk of V.N. Karazin Kharkiv National University series “Ecology”, (944(6), 61 – 71. Retrieved from http://journals.uran.ua/visnukkhnu_ecology/article/view/23273 (In Ukrainian).

Rules for monitoring and assessing the water quality of transboundary rivers. (1995). UN / ECE Work-ing Group on Monitoring and Assessment under the auspices of the Convention on the Protection and Use of Transboundary Watercourses and International Lakes. Bratislava.

DSTU 4166-2003. Water quality. Tests for growth inhibition of freshwater algae using Scenedesmus subspicatus and Selenastrum capricornutum (ISO 8692: 1998, MOD). (In Ukrainian).

Freshwater Algae and Cyanobacteria, Growth Inhibition Test.(2013). Test Chem .: Test No. 201. - OECD.

DSTU 4173-2003. Water quality. Determination of acute lethal toxicity to Daphnia magna Straus and Ceriodaphnia affinis Lilljeborg (Cladocera, Crustacea) (ISO 6341: 1996, MOD). (In Ukrainian).

DSTU 4174-2003. Water quality. Determination of sublethal and chronic toxicity of chemicals and water on Daphnia magna Straus and Ceriodaphnia affinis Lilljeborg (Cladocera, Crustacea) (ISO 1076: 2000, MOD). (In Ukrainian).

Daphnia sp. Acute Immobilisation Test. (2004). Test Chem.: Test No. 202. OECD.

Daphnia magna Reproduction Test. (2012). Test Chem.: Test No. 211. OECD.

DSTU 4074-2001 Water quality. Determination of acute lethal toxicity of chemicals and water in freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyrinidae)] Static method (ISO 7346-1: 1996, MOD). (In Ukrainian).

Fish, Acute Toxicity Test.(1992). Test Chem.: Test No. 203. OECD.

Fish, Juvenile Growth Test. (2000). Test Chem.: Test No. 215. OECD.

Published
2021-06-03
How to Cite
Krainiukova , A. M., Krainiukov , O. M., & Kryvytska , I. A. (2021). Use of Biotesting Methods for Assessing the Ecological Condition of Surface Waters. Visnyk of V. N. Karazin Kharkiv National University Series «Еcоlogy», (24), 103-116. https://doi.org/10.26565/1992-4259-2021-24-09

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