Novel squarylium dyes for detection of amyloid fibrils in vitro

  • K. O. Vus V.N. Karazin Kharkiv National University,Svobody Sq., 4, Kharkiv 61022, Ukraine
  • V. M. Trusova V.N. Karazin Kharkiv National University,Svobody Sq., 4, Kharkiv 61022, Ukraine
  • G. P. Gorbenko V.N. Karazin Kharkiv National University,Svobody Sq., 4, Kharkiv 61022, Ukraine
  • R. Sood Aalto University, Otakaari, 3, Еspoo FI-00076, Finland
  • P. Kinnunen Aalto University, Otakaari, 3, Еspoo FI-00076, Finland
Keywords: amyloid fibrils of insulin/lysozyme, binding model, quantum yield, squarylium dyes

Abstract

A series of novel symmetrical and asymmetrical squarylium dyes with the different substituents in the donor moieties have been tested for their ability to detect and characterize insulin and lysozyme amyloid fibrils prepared in acidic buffer at elevated temperature. The dye-protein binding parameters were estimated in terms of the one-site Langmuir adsorption model using the data of direct and reverse fluorimetric titrations. By comparing the dye quantum yields, binding affinities, and extents of the fluorescence enhancement in the protein-bound state, G6 and G7 were selected as the most prospective amyloid tracers. Furthermore, these probes provided evidence for the lower polarity of the lysozyme fibrillar grooves compared to insulin aggregates. The novel dyes G6 and G7 were recommended for amyloid fibril detection and characterization in the near-infrared region.

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Cited
How to Cite
Vus, K. O., Trusova, V. M., Gorbenko, G. P., Sood, R., & Kinnunen, P. (1). Novel squarylium dyes for detection of amyloid fibrils in vitro. Biophysical Bulletin, 2(32), 61-68. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1592
Section
Methods of biophysical investigations